Characterizing and optimizing magnetosome production of Magnetospirillum sp XM-1 isolated from Xi'an City Moat China.
Characterizing and optimizing magnetosome production of Magnetospirillum sp XM-1 isolated from Xi'an City Moat China.
复制标题
表征和优化从中国西安护城河中分离出的 Magnetospirillum sp XM-1 的磁小体生产。
DOI：
10.1093/femsle/fnv167
复制
发表时间：
2015
影响因子：
2.1
通讯作者：
Pan Yongxin
中科院分区：
文献类型：
--
作者：
Wang Yinzhao;Lin Wei;Li Jinhua;Zhang Tongwei;Li Ying;Tian Jiesheng;Gu Lixin;Heyden Yvan V;er;Pan Yongxin
趋磁细菌的纯培养是了解它们的生理、进化和生物矿化的理想方法。在此，我们报道了一种新的菌株--磁旋藻。XM-1是最近从富营养化的西安护城河中国分离培养出来的。用透射电子显微镜和岩石磁学相结合的方法对XM-1的磁小体生物矿化、结晶学和磁性进行了表征。采用响应面方法对菌体生长和磁铁矿产量进行了优化。我们发现，磁旋光菌株XM-1在磁铁矿磁小体、最适生长温度和营养需求等方面与模型菌株AMB-1不同。琥珀酸钠、硝酸钠和柠檬酸铁是影响XM-1细胞生长和磁小体生产的三个最重要的因素。
Pure culture of magnetotactic bacteria is desirable to understand their physiology, evolution and biomineralization. Here, we report a new strainMagnetospirillumsp. XM-1 that was recently isolated and cultivated from the eutrophic city moat of Xi'an, China. Magnetosome biomineralization, crystallographic and magnetic properties of XM-1 were characterized by using a combination of transmission electron microscopy and rock magnetic methods. Cell growth and magnetite production was optimized by response surface methodology. We found that theMagnetospirillumstrain XM-1 is different from the model strainMagnetospirillum magneticumAMB-1 in terms of magnetite magnetosomes, optimal growth temperature and nutrient requirements. Sodium succinate, sodium nitrate and ferric citrate are the three most significant factors associated with the optimization of cell growth and magnetosome production for XM-1.