Microscopy tools for the investigation of intracellular lipid storage and dynamics.
Microscopy tools for the investigation of intracellular lipid storage and dynamics.
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DOI：
10.1016/j.molmet.2015.12.005
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发表时间：
2016-03
影响因子：
8.1
通讯作者：
Hesselink MKC
中科院分区：
文献类型：
--
作者：
Daemen S;van Zandvoort MAMJ;Parekh SH;Hesselink MKC
脂质在异位组织如骨骼肌、肝脏和心脏中的过量储存似乎与代谢异常和心脏疾病密切相关。细胞内脂质储存发生在脂滴中，脂滴作为细胞代谢中的活性细胞器而受到关注。高分辨率显微镜和显微光谱学的最新发展为研究细胞内脂质的生理和生物化学开辟了新的途径。这篇综述的目的是概述最近的技术进展，在显微镜，其应用的可视化，识别和定量的细胞内脂质，特别关注脂滴。此外，我们试图总结目前可用于脂质可视化的探针。脂质探针的不断发展与显微技术的快速发展相结合，可以为细胞内脂质的作用和动力学提供新的见解。此外，细胞内脂质的原位鉴定现在是可能的，并有望增加一个新的维度的脂质生化分析，及其与（病理）生理学的关系。
Excess storage of lipids in ectopic tissues, such as skeletal muscle, liver, and heart, seems to associate closely with metabolic abnormalities and cardiac disease. Intracellular lipid storage occurs in lipid droplets, which have gained attention as active organelles in cellular metabolism. Recent developments in high-resolution microscopy and microscopic spectroscopy have opened up new avenues to examine the physiology and biochemistry of intracellular lipids. The aim of this review is to give an overview of recent technical advances in microscopy, and its application for the visualization, identification, and quantification of intracellular lipids, with special focus to lipid droplets. In addition, we attempt to summarize the probes currently available for the visualization of lipids. The continuous development of lipid probes in combination with the rapid development of microscopic techniques can provide new insights in the role and dynamics of intracellular lipids. Moreover, in situ identification of intracellular lipids is now possible and promises to add a new dimensionality to analysis of lipid biochemistry, and its relation to (patho)physiology.