Nanohydroxyapatite, Nanosilicate-Reinforced Injectable, and Biomimetic Gelatin-Methacryloyl Hydrogel for Bone Tissue Engineering.
Nanohydroxyapatite, Nanosilicate-Reinforced Injectable, and Biomimetic Gelatin-Methacryloyl Hydrogel for Bone Tissue Engineering.
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用于骨组织工程的纳米羟基磷灰石、纳米硅酸盐增强注射剂和仿生明胶-甲基丙烯酰水凝胶
DOI：
10.2147/ijn.s321387
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发表时间：
2021
影响因子：
8
通讯作者：
Cheng H
中科院分区：
文献类型：
--
作者：
Shi Z;Zhong Q;Chen Y;Gao J;Pan X;Lian Q;Chen R;Wang P;Wang J;Shi Z;Cheng H
鉴于自体骨移植治疗骨缺损受到供体骨供应不足和致病性的限制，开发仿生骨移植材料作为一种替代材料一直受到人们的关注。然而，在设计能够以快速方便的策略整合骨细胞外基质、成骨细胞和骨诱导成分的仿生特性的仿骨材料方面仍然存在障碍。方法设计并制备了一种装载间充质干细胞（MSC）、纳米羟基磷灰石（HAP）和纳米硅酸盐（SN）的骨组织工程模拟骨和注射明胶-甲基丙烯酰水凝胶（GelMA-HAP-SN）体系，系统地研究了GelMA-HAP-SN在体外和体内的成骨能力。结果羟基磷灰石的引入增强了水凝胶与天然骨细胞外基质的成分相似性，SN的加入使水凝胶具有可注射性和成骨性。结果，GelMA-HAP-SN水凝胶显示出细胞活力、增殖和扩散行为的增加。GelMA-HAP-SN水凝胶还扩增了嵌入MSCs的成骨生物标志物的表达和基质矿化。将msc包封的GelMA-HAP-SN水凝胶注射到大鼠临界尺寸的颅骨缺损中，显微ct和组织形态学染色结果进一步证实了其良好的骨再生能力。结论mscs - hap - sn水凝胶是一种有潜力的骨缺损移植材料。
Purpose Given that autologous bone graft for bone defects is limited by insufficient supply and morbidity at the donor site, developing biomimetic graft materials as an alternative has gained consistent attention. However, obstacles in designing bone-mimetic materials that could integrate the biomimetic nature of the bone extracellular matrix, osteogenic cells, and osteoinductive ingredients with a fast and convenient strategy still exist. Methods This study designed and fabricated a mesenchymal stem cell (MSC)-laden, nanohydroxyapatite (HAP), and nanosilicate (SN)-loaded bone mimetic and injectable gelatin-methacryloyl hydrogel (GelMA-HAP-SN) system for bone tissue engineering, and systemically investigated the osteogenic capacity of GelMA-HAP-SN in vitro and in vivo. Results Introducing HAP enhanced the compositional similarity to the natural bone extracellular matrix, and SN loading endowed the hydrogel with injectable and osteogenic ability. As a result, the GelMA-HAP-SN hydrogel demonstrated an increase in cellular viability, proliferation, and spreading behavior. The GelMA-HAP-SN hydrogel also amplified the embedded MSCs’ osteogenic biomarkers’ expression and matrix mineralization. Furthermore, the MSC-encapsulated GelMA-HAP-SN hydrogel was injected into rats’ critical-sized calvaria defect, and micro-CT and histomorphometry staining results further confirmed its excellent bone regeneration ability. Conclusion These MSC-loaded GelMA-HAP-SN hydrogels are potential graft materials for bone defect treatment.