Towards the application of Al2O3/ZnO nanolaminates in immunosensors: total internal reflection spectroscopic ellipsometry based evaluation of BSA immobilization
Towards the application of Al2O3/ZnO nanolaminates in immunosensors: total internal reflection spectroscopic ellipsometry based evaluation of BSA immobilization
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DOI：
10.1039/c8tc03091j
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发表时间：
2018-08-28
影响因子：
6.4
通讯作者：
Ramanaviciene, Almira
中科院分区：
文献类型：
--
作者：
Balevicius, Zigmas;Paulauskas, Andrius;Ramanaviciene, Almira
在这项研究中，Al 2 O3/ZnO纳米叠层可能的应用在光学免疫传感器的设计进行了评估。利用全内反射椭圆偏振法（TIRE）研究了在N-（3-氨丙基）三乙氧基硅烷（APTES）修饰的Al 2 O3/ZnO纳米层压板表面形成牛血清白蛋白（BSA）单分子膜的光学响应.评估了Al 2 O3和ZnO层的厚度对基于Al 2 O3/ZnO纳米叠层结构的性能的影响。这项研究表明，从Al 2 O3和ZnO之间的界面的光学响应的增强在全内反射配置的多次反射的显着贡献。基于四个50 nm厚的交替Al 2 O3和ZnO层的总厚度为200 nm的Al 2 O3/ZnO纳米层压材料显示出比基于两个100 nm氧化物层的纳米层压材料更好的灵敏度。椭圆偏振参数（）和（）的实时监测表明，BSA成功地共价连接到纳米层压/APTES表面。根据椭圆偏振参数（）和（）的实时数据评估固定化BSA的表面浓度。讨论了Al_2O_3/ZnO纳米叠层在光学免疫传感器中应用的优缺点。
In this research Al2O3/ZnO nanolaminates were evaluated for possible application in the design of optical immunosensors. Total internal reflection ellipsometry (TIRE) was utilized to study the optical response during the formation of a bovine serum albumin (BSA) based monolayer on the surface of Al2O3/ZnO nanolaminates, which were pre-modified with a N-(3-aminopropyl)triethoxysilane (APTES) layer. The influence of the thicknesses of Al2O3 and ZnO layers on the performance of Al2O3/ZnO nanolaminate based structures has been assessed. This research has shown the noticeable contribution of multiple reflections from the interfaces between Al2O3 and ZnO for the enhancement of the optical response in a total internal reflection configuration. Al2O3/ZnO nanolaminates of 200 nm total thickness based on four 50 nm thick alternating Al2O3 and ZnO layers have shown better sensitivity than the nanolaminate based on two 100 nm oxide-layers. Real-time monitoring of the ellipsometric parameters () and () has shown that BSA was successfully covalently attached to the nanolaminate/APTES surface. The surface concentration of immobilized BSA was evaluated from the real-time data of the ellipsometric parameters () and (). The expected advantages and disadvantages of Al2O3/ZnO nanolaminates during expected application in optical immunosensors are discussed.