Inhibition of fowlpox virus by an aqueous acetone extract from galls of Guiera senegalensis J. F. Gmel (Combretaceae)
Inhibition of fowlpox virus by an aqueous acetone extract from galls of Guiera senegalensis J. F. Gmel (Combretaceae)
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DOI：
10.1016/j.jep.2004.09.016
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发表时间：
2005-01-01
影响因子：
5.4
通讯作者：
Viljoen, GJ
中科院分区：
文献类型：
--
作者：
Lamien, CE;Meda, A;Viljoen, GJ
对塞内加尔圭埃拉虫瘿丙酮水提取物进行了体外抗鸡痘病毒活性筛选。使用细胞病变效应（CPE）抑制和空斑抑制试验来显示存在针对FPV的抗病毒作用，而细胞毒性试验确立了提取物对体外细胞的相对安全性。植物化学分析表明，存在的酚类化合物，包括类黄酮，单宁和花青素以及类固醇和生物碱。薄层色谱（TLC）分析也揭示了槲皮苷，槲皮素，山奈酚，芹菜素，芦丁，没食子酸以及未知的黄酮类化合物和未知的酚酸的存在。提取物的抗病毒作用部分归因于酚类成分，包括黄酮类化合物。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
An aqueous acetone extract from the galls of Guiera senegalensis was screened for in vitro antiviral activity against fowlpox virus (FPV). Cytopathic effect (CPE) inhibition and plaque inhibition assays were used to show presence of antiviral effects against FPV, whilst cytotoxicity assays established the relative safety of the extract for cells in vitro. Phytochemical analyses revealed the presence of phenolic compounds including flavonoids, tannins and anthocyanins as well as steroids and alkaloids. Thin-layer chromatographical (TLC) analysis also revealed the presence of quercitrin, quercetin, kaempferol, apigenin, rutin, gallic acid as well as unknown flavonoids and unknown phenolic acids. The antiviral effect of the extract was partially attributed to phenolic components including flavonoids. (C) 2004 Elsevier Ireland Ltd. All rights reserved.