Candida haemulonii complex: species identification and antifungal susceptibility profiles of clinical isolates from Brazil
Candida haemulonii complex: species identification and antifungal susceptibility profiles of clinical isolates from Brazil
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DOI：
10.1093/jac/dku321
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发表时间：
2015-01-01
影响因子：
5.2
通讯作者：
Santos, Andre L. S.
中科院分区：
文献类型：
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作者：
Ramos, Livia S.;Figueiredo-Carvalho, Maria Helena G.;Santos, Andre L. S.
目的：新出现的真菌病原体，包括念珠菌haemulonii复合体（念珠菌haemulonii，念珠菌haemulonii变种。念珠菌属和杜布沙木隆念珠菌属）的抗真菌耐药性是值得注意的。12个菌株与C. haemulonii是从巴西医院的患者身上回收的。在这里，我们的目的是通过分子方法，利用该真菌复合体的当前分类来鉴定这些分离株，并评估其抗真菌药敏谱。方法：通过真菌学方法重新检查真菌分离株以证明其认证，然后通过ITS1 - 5. 8S-ITS2基因测序进行表征。采用CLSI公布的微量肉汤稀释法（M27-A3/M27-S3）进行药敏试验。海缪龙。经ITS序列测定，5株分离物被鉴定为C. haemulonii、C. duobushaemulonii和C.黑穆隆原变种你好所有12株临床分离株均对红霉素B（MIC范围为2~> 16 mg/L）和氟康唑（MIC>= 64 mg/L）耐药。分离到一株C.黑穆隆原变种A和两个C. duobushaemulonii对伊曲康唑呈剂量依赖性，而其余菌株（75%）对该抗真菌剂耐药。12株分离株中有8株（66.7%）对伏立康唑耐药（MIC>= 16 mg/L），而所有分离株对卡泊芬净敏感（MIC
Objectives: The emerging fungal pathogens comprising the Candida haemulonii complex (Candida haemulonii, Candida haemulonii var. vulnera and Candida duobushaemulonii) are notable for their antifungal resistance. Twelve isolates with phenotypic similarity to C. haemulonii were recovered from patients in Brazilian hospitals. Here we aimed to identify these isolates by a molecular approach, using the current classification of this fungal complex, and to evaluate their antifungal susceptibility profiles.Methods: The fungal isolates were rechecked to certify their authentication by mycology methodologies and then characterized by ITS1-5.8S-ITS2 gene sequencing. A susceptibility assay was performed using the broth microdilution method published by CLSI (M27-A3/M27-S3).Results: Based on biochemical tests, all Brazilian isolates were identified as C. haemulonii. After employing ITS sequencing, five isolates were identified as C. haemulonii, four as C. duobushaemulonii and three as C. haemulonii var. vulnera. All 12 clinical isolates were resistant to amphotericin B (MICs ranged from 2 to > 16 mg/L) and fluconazole (MICs >= 64 mg/L). One isolate of C. haemulonii var. vulnera and two isolates of C. duobushaemulonii were susceptible-dose dependent to itraconazole, while the remaining isolates (75%) were resistant to this antifungal. Eight out of 12 isolates (66.7%) were resistant to voriconazole (MICs >= 16 mg/L), while all isolates were susceptible to caspofungin (MICs