Affimers and nanobodies as molecular probes and their applications in imaging.
Affimers and nanobodies as molecular probes and their applications in imaging.
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DOI：
10.1242/jcs.259168
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发表时间：
2022-07-15
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
抗体是标记细胞中分子的最广泛使用的传统工具。在过去的五到十年里，许多新的标记工具已经开发出来，与传统的抗体相比具有显着的优势。在这里，我们专注于纳米抗体和称为Affimers的非抗体结合支架蛋白。我们解释了它们是如何产生，选择和生产的，我们描述了它们的小尺寸，高结合亲和力和特异性如何为它们提供与抗体相比的许多优势。特别重要的是，它们的小尺寸使它们能够更好地穿透细胞以及组织内致密的细胞骨架区域，为它们提供超分辨率成像的特殊优势，因为它们将荧光团与几纳米的待成像目标蛋白质放置在一起。我们希望这些新工具能引起许多细胞生物学家的广泛兴趣，并期望它们成为超分辨率成像的首选工具。Affimers和nanobodies是小的结合蛋白，它们在细胞生物学中取代抗体得到了广泛的应用。我们解释了Affimers和nanobodies是如何产生的，使用的，以及它们在超分辨率成像中的优势。
Antibodies are the most widely used, traditional tool for labelling molecules in cells. In the past five to ten years, many new labelling tools have been developed with significant advantages over the traditional antibody. Here, we focus on nanobodies and the non-antibody binding scaffold proteins called Affimers. We explain how they are generated, selected and produced, and we describe how their small size, high binding affinity and specificity provides them with many advantages compared to antibodies. Of particular importance, their small size enables them to better penetrate dense cytoskeletal regions within cells, as well as tissues, providing them with specific advantage for super-resolution imaging, as they place the fluorophore with a few nanometres of the target protein being imaged. We expect these novel tools to be of broad interest to many cell biologists and anticipate them becoming the tools of choice for super-resolution imaging. Summary: Affimers and nanobodies are small binding proteins that are becoming widely used in cell biology in place of antibodies. We explain how Affimers and nanobodies are generated, used and their advantages for super-resolution imaging.
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影响因子：
16.6
作者：
Haza KZ;Martin HL;Rao A;Turner AL;Saunders SE;Petersen B;Tiede C;Tipping K;Tang AA;Ajayi M;Taylor T;Harvey M;Fishwick KM;Adams TL;Gaule TG;Trinh CH;Johnson M;Breeze AL;Edwards TA;McPherson MJ;Tomlinson DC
通讯作者：
Tomlinson DC
影响因子：
5.3
作者：
de Beer MA;Giepmans BNG
通讯作者：
Giepmans BNG
影响因子：
4.7
作者：
Keller, Laura;Bery, Nicolas;Olichon, Aurelien
通讯作者：
Olichon, Aurelien
DOI：
10.1073/pnas.90.22.10603
发表时间：
1993-11-15
影响因子：
11.1
作者：
GREENBERG, AS;STEINER, L;FLAJNIK, MF
通讯作者：
FLAJNIK, MF
影响因子：
10.8
作者：
Curd AP;Leng J;Hughes RE;Cleasby AJ;Rogers B;Trinh CH;Baird MA;Takagi Y;Tiede C;Sieben C;Manley S;Schlichthaerle T;Jungmann R;Ries J;Shroff H;Peckham M
通讯作者：
Peckham M