Brazilian propolis inhibits the differentiation of Th17 cells by inhibition of interleukin-6-induced phosphorylation of signal transducer and activator of transcription 3
Brazilian propolis inhibits the differentiation of Th17 cells by inhibition of interleukin-6-induced phosphorylation of signal transducer and activator of transcription 3
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DOI：
10.3109/08923973.2012.657304
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发表时间：
2012-10-01
影响因子：
3.3
通讯作者：
Masuzawa, Toshiyuki
中科院分区：
文献类型：
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作者：
Okamoto, Yoshihiro;Tanaka, Mayuri;Masuzawa, Toshiyuki
蜂胶是蜜蜂从各种植物的叶芽和树皮裂缝中收集的一种树脂物质。据报道，它具有免疫调节活性。此前，我们报道了巴西蜂胶乙醇提取物对实验性类风湿关节炎(RA)动物模型胶原诱导性关节炎(CIA)发病的保护作用。此外，我们还发现，对CIA的保护作用与抑制CIA小鼠产生IL-17有关。在本研究中，我们首次证实了蜂胶在体外抑制IL-6+转化生长因子-β诱导的Th17分化。蜂胶还抑制IL-6诱导的信号转导和转录激活因子3(STAT3)的磷酸化，STAT3是Th17发育过程中细胞因子激活的必要转录因子，同时还增强了参与下调STAT3磷酸化的细胞因子信号转导和转录激活因子3的表达抑制。这些数据表明，蜂胶对Th17分化的抑制作用可能有助于控制自身免疫性疾病中失衡的细胞因子网络。
Propolis is a resinous substance collected by honeybees from leaf buds and cracks in the bark of various plants. It has been reported to show immunomodulatory activity. Previously, we reported the protective effect of Brazilian propolis ethanolic extract against the pathogenesis of collagen-induced arthritis (CIA), an experimental animal model of rheumatoid arthritis (RA). Moreover, we found that the protective effect against CIA was involved in suppression of the production of interleukin-17 (IL-17) in CIA mice. In the present study, we demonstrated for the first time that propolis inhibited IL-6 plus transforming growth factor-beta induced Th17 differentiation in vitro. Propolis also suppressed the IL-6-induced phosphorylation of signal transducer and activator of transcription 3 (STAT3), a cytokine-activated essential transcription factor in Th17 development, concomitantly with the enhanced suppressor of cytokine signaling 3 expression involved in the downregulation of STAT3 phosphorylation. These data suggest that the suppressive effect of propolis on Th17 differentiation might be useful for controlling unbalanced cytokine networks in autoimmune diseases.