Use of (1-3)-β-D-glucan concentrations in dust as a surrogate method for estimating specific fungal exposures
Use of (1-3)-β-D-glucan concentrations in dust as a surrogate method for estimating specific fungal exposures
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DOI：
10.1111/j.1600-0668.2008.00526.x
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发表时间：
2008-06-01
期刊：
影响因子：
5.8
通讯作者：
Vesper, S.
中科院分区：
文献类型：
--
作者：
Iossifova, Y.;Reponen, T.;Vesper, S.
室内暴露于真菌与呼吸道症状有关，通常归因于其细胞壁成分（1-3）-β-D-葡聚糖。进行（1-3）-β-D-葡聚糖分析比真菌孢子的培养或显微镜计数耗时更少，劳动强度更低。这促使许多人使用（1-3）-β-D-葡聚糖作为真菌暴露的替代品。本研究的目的是检查哪些室内真菌物种是导致现场灰尘样本中（1-3）-β-D-葡聚糖浓度的主要因素。采用定量聚合酶链反应（QPCR）方法对297份室内灰尘样品中的36种真菌进行了分析。同时使用终点显色鲎变形细胞裂解物测定法分析这些样品的（1-3）-β-D-葡聚糖浓度。利用线性回归分析，然后进行因子分析和结构方程建模，以确定对现场灰尘样本中的（1-3）-β-D-葡聚糖浓度有主要贡献的真菌物种。研究表明，枝孢菌属和曲霉属以及黑附球菌、短密青霉菌和沃尔菌是这些家庭灰尘样本中（1-3）-β-D-葡聚糖含量的最重要贡献者。对（1-3）-β-D-葡聚糖浓度贡献最大的物种也是室内环境中最普遍的物种。然而，室内灰尘中常见的真菌种链格孢菌似乎不是（1-3）-β-D-葡聚糖的重要来源。
Indoor exposure to fungi has been associated with respiratory symptoms, often attributed to their cell wall component, (1-3)-beta-D-glucan. Performing (1-3)-beta-D-glucan analysis is less time consuming and labor intensive than cultivation or microscopic counting of fungal spores. This has prompted many to use (1-3)-beta-D-glucan as a surrogate for fungal exposure. The aim of this study was to examine which indoor fungal species are major contributors to the (1-3)-beta-D-glucan concentration in field dust samples. We used the quantitative polymerase chain reaction (QPCR) method to analyze 36 indoor fungal species in 297 indoor dust samples. These samples were also simultaneously analyzed for (1-3)-beta-D-glucan concentration using the endpoint chromogenic Limulus Amebocyte lysate assay. Linear regression analysis, followed by factor analysis and structural equation modeling, were utilized in order to identify fungal species that mostly contribute to the (1-3)-beta-D-glucan concentration in field dust samples. The study revealed that Cladosporium and Aspergillus genera, as well as Epicoccum nigrum, Penicillium brevicompactum and Wallemia sebi were the most important contributors to the (1-3)-beta-D-glucan content of these home dust samples. The species that contributed most to the (1-3)-beta-D-glucan concentration were also the most prevalent in indoor environments. However, Alternaria alternata, a common fungal species in indoor dust, did not seem to be a significant source of (1-3)-beta-D-glucan.