Characterization of avian leukosis virus subgroup J isolated between 1999 and 2013 in China
Characterization of avian leukosis virus subgroup J isolated between 1999 and 2013 in China
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1999年至2013年中国分离的禽白血病病毒J亚型的特征
DOI：
10.3382/ps/pey241
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发表时间：
2018-10-01
期刊：
影响因子：
4.4
通讯作者：
Cui, Zhizhong
中科院分区：
文献类型：
--
作者：
Li, Jianliang;Meng, Fanfeng;Cui, Zhizhong
禽白血病病毒亚组J（ALV-J）连续感染了白羽毛鸡，层母鸡，栽培的黄色鸡和本土鸡。在中国，感染率和肿瘤性吸引了越来越广泛的关注。为了澄清ALV-J的流行病学现象与包膜蛋白GP85的演变的相关性，比较了从1999年至2013年不同遗传背景的鸡分离的140个ALV-J菌株。分析并比较了GP85蛋白的同源性和140个ALV-J与原型菌株HPRS-103的遗传谱系关系，以及相同的ALV-J菌株与8种美国分离株之间的同源性。结果表明，来自不同遗传背景和不同年份的原型HPRS-103和ALV-J分离株之间GP85蛋白的同源性变异范围没有显着差异。然而，遗传谱系分析表明，从同一类型的鸡分离的病毒菌株在系统发育树上保持彼此近乎近距离，这意味着感染鸡和病毒菌株的遗传背景之间存在相关性。对氨基酸序列的进一步分析也发现了相似的结果，并发现在不同遗传背景的鸡中形成了独特的氨基酸位点，这证明ALV-J可以通过氨基酸变异适应新宿主。遗传序列系统发育树分析比评估ALV-J相关性的序列同源性比较更具代表性。这些结论有助于控制和预防ALV感染。 ALV-J在中国土著鸡中仍然很普遍，应给予更多注意以实现净化。
Avian leukosis virus subgroup J (ALV-J) has successively infected white feather chickens, layer hens, cultivated yellow chickens, and indigenous chickens; infection rates and tumorigenicity have attracted increasingly extensive attention in China. To clarify the correlation of the epidemiological phenomenon of ALV-J with the evolution of envelope protein gp85, 140 strains of ALV-J isolated from chickens with different genetic backgrounds from 1999 to 2013 were compared. The homology of the gp85 protein and genetic genealogical relationships between 140 strains of ALV-J and the prototype strain HPRS-103, as well as between the same ALV-J strains and 8 American isolates, were analyzed and compared. The results showed that there was no significant difference in the variation range of homology of the gp85 protein between the prototype HPRS-103 and ALV-J isolates from different genetic backgrounds and different years. However, genetic pedigree analysis showed that virus strains that isolated from the same type of chickens remained close to each other on the phylogenetic tree, which means that there was a correlation between the genetic background of infected chickens and virus strains. Further analysis of amino acid sequences also found similar results and revealed that unique amino acid sites were formed in chickens with different genetic backgrounds, which proved that ALV-J could adapt to the new host through amino acid variation. Genetic sequence phylogenetic tree analysis was more representative than sequence homology comparisons for assessing ALV-J correlations. These conclusions contributed to the control and prevention of ALV infection. ALV-J is still prevalent in Chinese indigenous chickens, more attentions should be given to fulfill the purification.