Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase
Nongenetic Reprogramming of a Fungal Highly Reducing Polyketide Synthase
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DOI：
10.1021/ja204200x
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发表时间：
2011-07-20
影响因子：
15
通讯作者：
Cox, Russell J.
中科院分区：
文献类型：
--
作者：
Yakasai, Ahmed A.;Davison, Jack;Cox, Russell J.
在表观遗传修饰剂5-氮杂胞苷和辛二酰双异羟肟酸的存在下，以及在来自tenellin生物合成基因簇的单个基因被沉默的条件下，研究了来自球孢白僵菌CBS110.25的真菌代谢物tenellin的生物合成。许多新的化合物被合成，表明正常的主要生物合成的tenellin只是一个结果的各种各样的可能的产品。产物的结构揭示了关于tenellin聚酮合酶的编程选择性的关键线索。
The biosynthesis of the fungal metabolite tenellin from Beauveria bassiana CBS110.25 was investigated in the presence of the epigenetic modifiers 5-azacytidine and suberoyl bis-hydroxamic acid and under conditions where individual genes from the tenellin biosynthetic gene cluster were silenced. Numerous new compounds were synthesized, indicating that the normal predominant biosynthesis of tenellin is just one outcome out of a diverse array of possible products. The structures of the products reveal key clues about the programming selectivities of the tenellin polyketide synthase.