The influence of tamoxifen on growth behavior and cell-cell adhesion in OSCC in vitro
The influence of tamoxifen on growth behavior and cell-cell adhesion in OSCC in vitro
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DOI：
10.1016/j.oraloncology.2006.09.005
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发表时间：
2007-08-01
期刊：
影响因子：
4.8
通讯作者：
Lage, Herrmann
中科院分区：
文献类型：
--
作者：
Nelson, Katja;Helmstaedter, Victor;Lage, Herrmann
本研究旨在探讨三苯氧胺对口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）细胞生长和聚集行为的影响，重点研究E-cadherin和beta-catenin的表达模式。用不同浓度的他莫昔芬处理口腔鳞状细胞癌细胞系（UM-SCC-14 A、UM-SCC-14 B和UM-SCC-14 C）。分析其生长和聚集行为以及蛋白质表达及其变化。所有细胞均为雌激素受体（ER）阳性。他莫昔芬诱导显著的生长抑制并诱导形成细胞聚集体的能力。这种现象并不伴随着E-钙粘蛋白或β-连环蛋白表达的变化或由于转录的变化。β-连环蛋白在所有细胞系中显示出分离的膜染色和核分布。在UM-SCC-14 C中观察到有缺陷的E钙粘蛋白/β-连环蛋白复合物，通过他莫昔芬治疗没有恢复。在所有细胞系中，细胞-细胞形成增加，而E-钙粘蛋白或β-连环蛋白的功能和数量状态无任何改变，表明不涉及经典E-钙粘蛋白/β-连环蛋白的新型细胞-细胞粘附复合物影响OSCC中的细胞生长和细胞间粘附。(C)2006爱思唯尔有限公司保留所有权利。
The aim of this study was to evaluate the influence of tamoxifen on the growth and aggregation behavior, focusing on the expression pattern of E-cadherin and beta-catenin, in oral squamous cell carcinoma (OSCC) in vitro. Oral squamous cancer cell lines (UM-SCC-14A, UM-SCC-14B and UM-SCC-14C) were treated with various concentrations of tamoxifen. Growth and aggregation behavior as well as the protein expression and its changes were analysed. All cell tines are estrogen receptor (ER) positive. Tamoxifen induced a significant growth inhibition and induced the ability to form cell aggregates. This phenomena was not accompanied by a change in E-cadherin or beta-catenin expression or due to transcriptional changes. beta-catenin showed isolated membrane staining and nuclear distribution in all cell lines. A defective Ecadherin/beta-catenin complex was seen in UM-SCC-14C with no restoration through tamoxifen treatment. The cell-cell formation is increased in all cell lines without any alterations in the functional and quantitative status of E-cadherin or beta-catenin, indicating that novel cell-cell adhesion complexes not involving the classical E-cadherin/beta-catenin influence cell growth and intercellular adhesion in OSCC. (C) 2006 Elsevier Ltd. All rights reserved.