Rapid and Efficient Isolation of High Quality Nucleic Acids from Plant Tissues Rich in Polyphenols and Polysaccharides
Rapid and Efficient Isolation of High Quality Nucleic Acids from Plant Tissues Rich in Polyphenols and Polysaccharides
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DOI：
10.1007/s12033-011-9384-8
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发表时间：
2011-10-01
影响因子：
2.6
通讯作者：
Garoosi, Ghasem-Ali
中科院分区：
文献类型：
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作者：
Japelaghi, Reza Heidari;Haddad, Raheem;Garoosi, Ghasem-Ali
从富含多糖和多酚的植物组织中分离高质量的核酸往往是困难的。这些物质的存在会影响分离出的核酸的质量和/或数量。在这里，我们描述了一种快速高效的核酸提取方案，与其他测试方法相比，它可以有效地从富含多糖和多酚化合物的植物组织中纯化出高质量的核酸，例如不同的葡萄组织和果树的果实组织。该方案分离的核酸成功地用于许多功能基因组的实验，包括聚合酶链反应，逆转录聚合酶链反应（RT-PCR），克隆和半定量RT-PCR。
Isolation of high quality nucleic acids from plant tissues rich in polysaccharides and polyphenols is often difficult. The presence of these substances can affect the quality and/or quantity of the nucleic acids isolated. Here, we describe a rapid and efficient nucleic acids extraction protocol that in contrast to other methods tested, effectively purify high quality nucleic acids from plant tissues rich in polysaccharides and polyphenolic compounds such as different grape tissues and fruit tissue of fruit trees. The nucleic acids isolated with this protocol were successfully used for many functional genomic based experiments including polymerase chain reaction, reverse transcription polymerase chain reaction (RT-PCR), cloning, and semiquantitative RT-PCR.