Initiation of protein synthesis in vitro by a clostridial system. I. Specificity in the translation of natural messenger ribonucleic acids.
Initiation of protein synthesis in vitro by a clostridial system. I. Specificity in the translation of natural messenger ribonucleic acids.
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梭菌系统在体外启动蛋白质合成。
DOI：
10.1016/s0021-9258(19)44030-1
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发表时间：
1973
影响因子：
4.8
通讯作者：
J. Rabinowitz
中科院分区：
文献类型：
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作者：
M. Stallcup;J. Rabinowitz
从巴氏梭菌中提取的信使RNA能通过核糖体和来源于C的启动因子刺激体外蛋白质合成。大肠埃希氏菌的巴氏杆菌。检测的其他信使有：F2RNA、T4早期信使RNA等。ColimRNA，与核糖体和源于核糖体的启动因子一起发挥活性。Coli，但不能与来源于C。巴氏杆菌。通过盐洗核糖体与这两种细菌的启动因子在蛋白质合成实验中的异源组合，证明了盐洗核糖体而不是启动因子控制了mRNA翻译的特异性，并决定了蛋白质合成的最适特征镁。启动因素影响每种类型的信使核糖核酸的翻译效率。使用时观察到的翻译的相对效率。大肠埃希氏菌引发因子和使用C。巴氏杆菌的启动因子因mRNA类型的不同而不同。盐洗核糖体和来自两个细菌物种的启动因子的异源组合也被测试了它们与fMet-tRNA结合的能力，以响应f2 RNA、T4早期mRNA和ANDC。巴氏杆菌mRNA.这些检测表明，两个核糖体系统之间的信使特异性与蛋白质合成检测中发现的相同。此外，结果表明，与蛋白质合成分析相一致的是，盐洗的核糖体而不是启动因子控制了mRNA识别的特异性。
A messenger RNA preparation fromClostridium pasteurianumis active in stimulating protein synthesisin vitroby ribosomes and initiation factors derived fromC. pasteurianumor fromEscherichia coli. Other messengers tested, f2 RNA, T4 early mRNA, andE. colimRNA, are active with ribosomes and initiation factors derived fromE. coli, but not with those derived fromC. pasteurianum. By testing the heterologous combinations of salt-washed ribosomes and initiation factors from these two bacterial species in protein synthesis assays, it was demonstrated that the salt-washed ribosomes, and not the initiation factors, control the specificity of mRNA translation and also determine the characteristic magnesium optimum for protein synthesis. The initiation factors influence the efficiency of translation obtained with each type of mRNA. The relative efficiency of translation observed when usingE. coliinitiation factors and when usingC. pasteurianuminitiation factors varies with the different types of mRNA.The heterologous combinations of salt-washed ribosomes and initiation factors from the two bacterial species were also tested for their ability to bind fMet-tRNA in response to f2 RNA, T4 early mRNA, andC. pasteurianummRNA. These assays demonstrated the same difference in messenger specificities between the two ribosomal systems as was found in the protein synthesis assays. Moreover, the results indicated, in agreement with the protein synthesis assays, that the salt-washed ribosomes, and not the initiation factors, control the specificity of mRNA recognition.