Generation and scavenging of reactive oxygen species in wheat flag leaves under combined shading and waterlogging stress.
Generation and scavenging of reactive oxygen species in wheat flag leaves under combined shading and waterlogging stress.
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DOI：
10.1071/fp11165
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发表时间：
2012-02
期刊：
影响因子：
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通讯作者：
Huawei Li;J. Cai;Fulai Liu;D. Jiang;T. Dai;W. Cao
中科院分区：
文献类型：
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作者：
Huawei Li;J. Cai;Fulai Liu;D. Jiang;T. Dai;W. Cao
小麦（Triticum aestivum L.）在开花后0-7、8-15、16-23和24-31天对植株进行淹水和遮荫的联合处理。花后0-7、8-15、16-23和24-31天的WS分别造成产量损失17.18%、14.98%、7.93%和7.05%。这些损失与花后光合产物积累和千粒重的减少有关。WS降低了净光合速率（Pn）、暗适应下PSII光化学最大效率、实际光合效率和光化学猝灭系数，但提高了猝灭量子效率。WS引起的丙二醛和H2 O2的浓度增加，并增加超氧阴离子的释放。超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性在伤后4d（0 - 7、8-15和16-23 DAA）开始升高，在伤后8d（0-7、8-15和16-23 DAA）开始下降。抗坏血酸过氧化物酶、谷胱甘肽还原酶、脱氢抗坏血酸还原酶和单脱氢抗坏血酸还原酶活性在花后0-7、8-15和16-23天均呈上升趋势。花后16-24天，叶片Pn、活性氧水平和抗氧化酶活性在花后0-7天完全恢复，而在花后8-15天仅部分恢复。光合响应基因RcaB和Cab以及抗氧化酶相关基因Mn-SOD、Cu/Zn-SOD、CAT和GR的表达与Pn和抗氧化酶活性表现一致。
Wheat (Triticum aestivum L.) plants were subjected to combined waterlogging and shading (WS) at 0-7, 8-15, 16-23 and 24-31 days after anthesis (DAA). WS at 0-7, 8-15, 16-23 and 24-31 DAA caused a yield loss of 17.18%, 14.98%, 7.93% and 7.05%, respectively. These losses were related to reductions in post-anthesis photoassimilate accumulation and 1000-kernel weight. WS reduced net photosynthetic rate (Pn), the maximum efficiency of PSII photochemistry under dark adaptation, actual photosynthetic efficiency and the photochemical quenching coefficient, but increased the quantum yield of quenching. WS caused enhanced concentrations of malondialdehyde and H2O2, and an increased superoxide anion release. Superoxide dismutase and catalase activity were stimulated at 4 days after the onset of WS at 0-7 and 8-15 DAA, but decreased at 8 days after the onset of WS at 0-7, 8-15 and 16-23 DAA. Ascorbate peroxidase, glutathione reductase, dehydroascorbate reductase and monodehydroascorbate reductase activity increased during 0-8 days after the onset of WS at 0-7, 8-15 and 16-23 DAA. At 16-24 DAA, Pn, the level of reactive oxygen species and activity of the antioxidative enzymes fully recovered in plants subjected to WS at 0-7 DAA, but only partially recovered under WS at 8-15 DAA. Expression of the photosythesis-responsive genes RcaB and Cab, and the antioxidative enzyme-related genes Mn-SOD, Cu/Zn-SOD, CAT and GR were consistent with the performance of Pn and the activity of the antioxidative enzymes.