CYTOGENETIC AND IMMUNOLOGICAL IDENTIFICATION OF CLONAL EXPANSION OF STEM-CELLS INTO T-LYMPHOCYTES AND B-LYMPHOCYTES IN ONE ATOMIC-BOMB SURVIVOR
CYTOGENETIC AND IMMUNOLOGICAL IDENTIFICATION OF CLONAL EXPANSION OF STEM-CELLS INTO T-LYMPHOCYTES AND B-LYMPHOCYTES IN ONE ATOMIC-BOMB SURVIVOR
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DOI：
10.1182/blood.v86.6.2106.bloodjournal8662106
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发表时间：
1995-09-15
期刊：
影响因子：
20.3
通讯作者：
AKIYAMA, M
中科院分区：
文献类型：
--
作者：
KUSUNOKI, Y;KODAMA, Y;AKIYAMA, M
在受辐射照射的人中，外周血淋巴细胞中的染色体畸变频率升高，在同一个血液样本中以及在来自同一捐赠者的不同样本中经常遇到相同的核型变化。这样的克隆增殖来源于能够扩增并分化成几种细胞类型的单个未成熟干细胞，或者来源于能够在体内抗原刺激后扩增的单个成熟淋巴细胞。本研究从一名原子弹爆炸幸存者外周血淋巴细胞中分离出71个T淋巴细胞集落和58个B淋巴细胞集落。近10%的集落（6个T淋巴细胞和7个B淋巴细胞集落）显示相同的染色体异常。T细胞受体或IG重链基因的Southern印迹分析显示T淋巴细胞和B淋巴细胞集落之间分别存在不同的重排模式。因此，染色体畸变发生在分化成T和B谱系之前的前体细胞中，并且不是源自外周成熟T或B淋巴细胞的单克隆增殖。为了证实这一问题，通过染色体涂抹法检查了红系爆发形成单位（BFU-E）集落的细胞。两个易位，一个在5号和13号染色体之间，另一个在4号染色体和C组染色体之间，完全符合t（4;6），t（5; 13），在约10%的细胞中发现，结果表明，成年人的单个干细胞能够产生长-活的骨髓和淋巴后代，占循环淋巴细胞和造血祖细胞总数的百分之几。(C)1995年，美国血液学会。
Chromosome aberration frequency in peripheral blood lymphocytes is elevated in radiation-exposed people, and identical karyotypic changes are not infrequently encountered in one blood sample as well as in separate samples from the same donor. Such clonal propagation originates either from a single immature stem cell able to expand and differentiate into several cell types or from a single mature lymphocyte able to expand after antigen stimulation in vivo. In the present study, a total 71 T-lymphocyte and 58 B-lymphocyte colonies were established from one atomic-bomb survivor, who showed a persistent clonal aberration t(4;6), t(5;13) in phytohemagglutinin culture of peripheral lymphocytes. Nearly 10% of the colonies (6 T-lymphocyte and 7 B-lymphocyte colonies) showed the same chromosome abnormality, Southern blot analyses of the T-cell-receptor or Ig heavy-chain gene showed all different rearrangement patterns among T- or B-lymphocyte colonies, respectively. Thus, the chromosome aberration occurred in a precursor cell before differentiation into T and B lineages and was not derived from monoclonal proliferation of mature T or B lymphocytes in the periphery. To confirm the issue, cells from erythroid burst-forming unit (BFU-E) colonies were examined by the chromosome-painting method. Two translocations, one between chromosomes 5 and 13 and the other between chromosomes 4 and one of group C, perfectly consistent with the t(4;6), t(5;13), were found in about 10% of the cells, The results imply that a single stem cell of an adult is capable of generating long-lived myeloid and lymphoid progeny amounting to several percent of the total population of circulating lymphocytes and hematopoietic progenitors. (C) 1995 by The American Society of Hematology.