Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
Identification and Evaluations of Novel Insecticidal Proteins from Plants of the Class Polypodiopsida for Crop Protection against Key Lepidopteran Pests
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DOI：
10.3390/toxins11070383
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发表时间：
2019-07-01
期刊：
影响因子：
4.2
通讯作者：
Wu, Gusui
中科院分区：
文献类型：
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作者：
Liu, Lu;Schepers, Eric;Wu, Gusui
各种鳞翅目昆虫是造成全球主要作物损失的原因。虽然开发表达苏云金芽孢杆菌（Bt）蛋白的作物品种在控制主要鳞翅目害虫的损害方面是有效的，但一些昆虫种群已经进化为对某些Bt蛋白不敏感。在这里，我们报告了一个家族的同源蛋白质的发现，其中两个我们已经指定IPD083Aa和IPD083Cb，这是从铁线蕨属物种。这两种蛋白质与其他蛋白质没有已知的肽结构域、序列基序或特征。分别表达IPD083Aa或IPD083Cb的转基因大豆或玉米植物在田间条件下显示出免受几种关键害虫的摄食损害的保护。与目前转基因作物中使用的主要Bt蛋白的比较研究的结果表明，IPD 083蛋白通过结合不同的靶位点发挥功能。这些结果表明IPD 083 Aa和IPD 083 Cb可以作为传统基于BT的昆虫控制性状的替代品，具有对抗昆虫对BT蛋白的抗性的潜力。
Various lepidopteran insects are responsible for major crop losses worldwide. Although crop plant varieties developed to express Bacillus thuringiensis (Bt) proteins are e ff ective at controlling damage from key lepidopteran pests, some insect populations have evolved to be insensitive to certain Bt proteins. Here, we report the discovery of a family of homologous proteins, two of which we have designated IPD083Aa and IPD083Cb, which are from Adiantum spp. Both proteins share no known peptide domains, sequence motifs, or signatures with other proteins. Transgenic soybean or corn plants expressing either IPD083Aa or IPD083Cb, respectively, show protection from feeding damage by several key pests under field conditions. The results from comparative studies with major Bt proteins currently deployed in transgenic crops indicate that the IPD083 proteins function by binding to di ff erent target sites. These results indicate that IPD083Aa and IPD083Cb can serve as alternatives to traditional Bt-based insect control traits with potential to counter insect resistance to Bt proteins.