Lipoteichoic acid and Toll-like receptor 2 internalization and targeting to the Golgi are lipid raft-dependent
Lipoteichoic acid and Toll-like receptor 2 internalization and targeting to the Golgi are lipid raft-dependent
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DOI：
10.1074/jbc.m400466200
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发表时间：
2004-09-24
影响因子：
4.8
通讯作者：
Triantafilou, K
中科院分区：
文献类型：
--
作者：
Triantafilou, M;Manukyan, M;Triantafilou, K
脂壁酸（LTA）是革兰氏阳性菌的关键细胞壁成分，其功能与革兰氏阴性菌的脂多糖非常相似。已经证明LTA结合CD14并通过toll样受体2触发激活，但激活是否发生在细胞表面或内化是触发信号的必要条件尚未得到证实。在这项工作中，我们研究了LTA的结合和内化，发现LTA及其受体分子在脂筏中积累，随后迅速靶向高尔基体。这种内化似乎是脂质筏依赖的，因为破坏筏的药物抑制了LTA/ toll样受体2在高尔基体中的共定位。与脂多糖类似，LTA激活发生在细胞表面，并且观察到的运输是独立于信号传导的。
Lipoteichoic acid (LTA), a key cell wall component of Gram-positive bacteria, seems to function as an immune activator with characteristics very similar to lipopolysaccharide from Gram-negative bacteria. It has been shown that LTA binds CD14 and triggers activation via Toll-like receptor 2, but whether the activation occurs at the cell surface or internalization is required to trigger signaling has yet to be demonstrated. In this work we have investigated LTA binding and internalization and found that LTA and its receptor molecules accumulate in lipid rafts and are subsequently targeted rapidly to the Golgi apparatus. This internalization seems to be lipid raft-dependent because raft-disrupting drugs inhibited LTA/Toll-like receptor 2 colocalization in the Golgi. Similarly to lipopolysaccharide, LTA activation occurs at the cell surface, and the observed trafficking is independent of signaling.