The Home Observation of Periconceptional Exposures (HOPE) study, a prospective cohort: aims, design, recruitment and compliance.
The Home Observation of Periconceptional Exposures (HOPE) study, a prospective cohort: aims, design, recruitment and compliance.
复制标题
DOI：
10.1186/s12940-016-0153-9
复制
发表时间：
2016-06-08
期刊：
影响因子：
--
通讯作者：
Stanford JB
中科院分区：
文献类型：
--
作者：
Porucznik CA;Cox KJ;Schliep KC;Wilkins DG;Stanford JB
为了检查短暂的环境暴露及其与人类生殖力的关系，最好在夫妇双方受孕时进行暴露评估。我们进行了一项观察性的前瞻性队列研究，对一对试图怀孕的异性恋夫妇的两名成员进行了生物监测。夫妇收集尿液，唾液和精液标本，最多两个月经周期的日期对应的时间窗口受精，植入和早期妊娠，根据妇女的观察，她的宫颈液确定。2011年至2015年期间，有309对符合条件的夫妇接受了筛查，其中183对登记。11对夫妇（6.0%）退出或失访。最成功和最具成本效益的招聘策略是口碑（40%的参与夫妇），海报和传单（37%），以及有针对性的Facebook广告（13%），每对夫妇的招聘总投资为37.35美元。男性和女性均采集了≥ 97.2%的要求唾液样本，男性采集了≥ 89.9%的要求精液样本。在排卵期（±3天）内，97.1%的周期中女性至少采集了一份尿液标本，91.7%的周期中男性至少采集了一份尿液标本。排卵期尿液样本的每日依从性范围为女性66.5 - 92.4%，男性55.7 - 75.0%。在指定时间点完成问卷的依从性≥ 88%。计划怀孕的夫妇可以成功招募围受孕期监测，并将遵守密集的研究方案，包括家庭收集生物标本和问卷调查数据。
To examine transient environmental exposures and their relationship with human fecundity, exposure assessment should occur optimally at the time of conception in both members of the couple. We performed an observational, prospective cohort study with biomonitoring in both members of a heterosexual couple trying to conceive. Couples collected urine, saliva, and semen specimens for up to two menstrual cycles on days corresponding to the time windows of fertilization, implantation, and early pregnancy, identified based on the woman’s observations of her cervical fluid. Three hundred nine eligible couples were screened between 2011 and 2015, of which 183 enrolled. Eleven couples (6.0 %) withdrew or were lost to follow up. The most successful and cost effective recruiting strategies were word of mouth (40 % of participating couples), posters and flyers (37 %), and targeted Facebook advertising (13 %) with an overall investment of $37.35 spent on recruitment per couple. Both men and women collected ≥97.2 % of requested saliva samples, and men collected ≥89.9 % of requested semen samples. Within the periovulatory days (±3 days), there was at least one urine specimen collected by women in 97.1 % of cycles, and at least one by men in 91.7 % of cycles. Daily compliance with periovulatory urine specimens ranged from 66.5 to 92.4 % for women and from 55.7 to 75.0 % for men. Compliance was ≥88 % for questionnaire completion at specified time points. Couples planning to conceive can be recruited successfully for periconceptional monitoring, and will comply with intensive study protocols involving home collection of biospecimens and questionnaire data.