CLINICAL USEFULNESS OF A HIGHLY SENSITIVE ENZYME‐IMMUNOASSAY OF TSH
CLINICAL USEFULNESS OF A HIGHLY SENSITIVE ENZYME‐IMMUNOASSAY OF TSH
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高灵敏度 TSH 酶免疫测定法的临床实用性
DOI：
10.1111/j.1365-2265.1987.tb00833.x
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发表时间：
1987
影响因子：
3.2
通讯作者：
H. Ogawa
中科院分区：
文献类型：
--
作者：
Tomohiko Mori;Hiroo Imura;S. Bito;Katsuji Ikekubo;S. Inoue;S. Hashida;E. Ishikawa;H. Ogawa
用新近建立的TSH敏感的酶免疫分析法(EIA)测定了正常人和各种甲状腺疾病患者的血清TSH水平。该方法的最低检测浓度小于0.005 mU/L，与放射免疫法测定的TSH值具有良好的相关性(r=0.932)。26例正常人血清TSH范围为0.30~2~65mU/L，几何平均值为0.97RAU/L。27例初治甲亢患者中，除1例外，其余患者均检出促甲状腺激素，范围为0.005~0.19mU/L，几何均数为0.040。通过吸收、恢复、交叉反应和稀释研究，这些值被证明是人TSH的特异性；也排除了非特异性血清影响。10例破坏性甲状腺炎患者TSH值相近(0.005~0.20mU/L，平均0.032)。然而，在这些甲状腺功能亢进的情况下，TSH和循环甲状腺激素水平之间没有明显的相关性。此外，9名未经治疗的Graves‘s患者一次静脉注射都没有反应。注射TRH。13例甲状腺功能减退合并下丘脑-垂体疾病患者中，10例基础TSH<0.4mU/L，5例TRH峰值>0.8mU/L，与血清T_3显著升高有关。在3例希恩综合征患者中，观察到基础TSH水平升高。其中两人对TRH有反应，但这些TSH升高与T3升高无关。结论：这种灵敏的EIA可以检测大多数甲亢和中枢性甲状腺功能减退患者的TSH水平。然而，这些测量值的真正意义需要通过未来的研究来阐明。
Using a recently developed sensitive enzyme immunoassay (EIA) for TSH, serum TSH levels in normal subjects and patients with various thyroid disorders were measured. The minimum detectable concentration was less than 0.005 mU/l. An excellent correlation was observed between TSH values measured by EIA and by sensitive radioimmunoassay (r= 0.932). In 26 normal subjects, serum TSH ranged from 0.30 to 2‐65 mU/l (geometric mean 0.97 raU/l). In 27 untreated hyperthyroid Graves' patients, serum TSH was detected in all but one, ranging from 0.005 to 0.19 mU/l (geometric mean 0.040). These values were proved to be specific for human TSH by absorption, recovery, cross‐reactivity and dilution studies; non‐specific serum effects were also ruled out. In 10 patients with destructive thyroiditis, similar TSH values (0.005‐0.20 mU/l, mean 0.032) were observed. However, no significant correlations between TSH and circulating thyroid hormone levels were observed in these thyrotoxic conditions. Furthermore, 9 untreated Graves' patients did not respond to a single i.v. injection of TRH. In 13 hypothyroid patients with hypothalamopituitary disorders, 10 had basal TSH levels of less than 0.4 mU/l, and TRH tests in five gave peak TSH values of more than 0.8 mU/l, which were associated with significant increases in serum T3. In three patients with Sheehan's syndrome, elevated basal TSH levels were observed. Two of them responded to TRH, but these TSH elevations were not associated with T3 increases. In conclusion, this sensitive EIA could measure TSH levels in most patients with thyrotoxicosis and central hypothyroidism. However, the true significance of these measured values needs to be elucidated by future studies.
含有促甲状腺免疫球蛋白 (TSI) 的血清中的 γ 球蛋白与 125I-人 TSH 结合。
影响因子：
6.1
作者：
Beall,GN;Kruger,SR
通讯作者：
Kruger,SR