Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin
Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin
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DOI：
10.1126/science.280.5363.593
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发表时间：
1998-04-24
期刊：
影响因子：
56.9
通讯作者：
Stillman, B
中科院分区：
文献类型：
--
作者：
Zou, L;Stillman, B
CDC45P是一种启动DNA复制的蛋白质，在细胞周期的G(1)末期“启动”后和细胞周期的S阶段与染色质结合。CDC45P与染色质的结合依赖于ClB-CDC28激酶的活性以及功能上的CDC6P和Mcm2p，这表明CDC45P与S期细胞周期蛋白依赖性蛋白激酶(CDKs)激活后的复制前复合体有关。时间和对CDK的依赖表明，CDC45P与染色质的结合是启动DNA复制的关键，在S期，CDC45P与染色质上的微染色体维持蛋白发生物理作用，但CDC45P从染色质上解离的速度慢于染色质上的微染色体维持蛋白，这表明蛋白质的释放机制不同。
Cdc45p, a protein essential for initiation of DNA replication, associates with chromatin after "start" in late G(1) and during the S phase of the cell cycle. Binding of Cdc45p to chromatin depends on Clb-Cdc28 kinase activity as well as functional Cdc6p and Mcm2p, which suggests that Cdc45p associates with the prereplication complex after activation of S-phase cyclin-dependent kinases (CDKs). As indicated by the timing and the CDK dependence, binding of Cdc45p to chromatin is crucial for commitment to initiation of DNA replication, During S phase, Cdc45p physically interacts with minichromosome maintenance (MCM) proteins on chromatin; however, dissociation of Cdc45p from chromatin is slower than that of MCMs, which indicates that the proteins are released by different mechanisms.