Desorption Electrospray Ionization Mass Spectrometry Imaging for Tissue Analysis
Desorption Electrospray Ionization Mass Spectrometry Imaging for Tissue Analysis
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DOI：
10.1002/9780470027318.a9640
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发表时间：
2020-12
期刊：
影响因子：
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通讯作者：
Xiaowei Song;Hao Chen
中科院分区：
文献类型：
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作者：
Xiaowei Song;Hao Chen
质谱成像（MSI）已经成为质谱（MS）的重要技术，其通过分子质量来可视化分子（诸如药物、代谢物、脂质和蛋白质）的空间分布。解吸电喷雾电离质谱（DESI-MS）作为一种代表性的环境电离方法，只需极少或无需样品制备，已被证明在MSI中具有独特的优势。本文概述了这种强大的DESI-MSI技术，包括其基本原理，仪器，样品制备，方法优化，2D和3D成像，定量，统计分析，软件及其在制药，临床，生物医学，植物，微生物和法医研究中的应用。
Mass spectrometry imaging (MSI) has become a significant technique of mass spectrometry (MS) to visualize the spatial distribution of molecules such as drugs, metabolites, lipids, and proteins by their molecular masses. As a representative ambient ionization method that requires minimal or no sample preparation, desorption electrospray ionization mass spectrometry (DESI‐MS) has been shown to have its unique strength in MSI. This article provides an overview of this powerful DESI‐MSI technique, including its basic principle, instrumentation, sample preparation, method optimization, 2D and 3D imaging, quantitation, statistical analysis, software, and its applications in pharmaceutical, clinical, biomedical, plant, microbial, and forensic researches.