Proto-oncogene mRNA levels and activities of multiple transcription factors in C3H 10T1/2 murine embryonic fibroblasts exposed to 835.62 and 847.74 MHz cellular phone communication frequency radiation
Proto-oncogene mRNA levels and activities of multiple transcription factors in C3H 10T1/2 murine embryonic fibroblasts exposed to 835.62 and 847.74 MHz cellular phone communication frequency radiation
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DOI：
10.2307/3579942
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发表时间：
1999-03-01
影响因子：
3.4
通讯作者：
Hunt, CR
中科院分区：
文献类型：
--
作者：
Goswami, PC;Albee, LD;Hunt, CR
这项研究旨在确定在移动电话通信中通常使用的类型的不同调制的射频是否会在生物系统中引发一般的应激反应。研究的两种调制和频率是载波频率为835.62兆赫的频繁调制连续波和以847.74兆赫为中心的码分多址调制。原癌基因表达的变化，通过测量For，Jun和Myc mRNA水平以及AP1，AP2和NF-kappa B转录因子的DNA结合活性来确定，作为一般应激反应的指标，评估了射频暴露对原癌基因表达的影响：(1)在指数生长的C3H10T1/2小鼠胚胎成纤维细胞向平台期过渡期间和(2)在血清刺激后无血清细胞向增殖周期过渡期间。用835.62 MHz快速连续波或847.74 MHz码分多址微波(平均比吸收率为0.6W/kg)照射无血清的细胞，并未显著改变血清刺激后原癌基因表达的动力学，类似地，这些暴露也不影响指数型细胞在过渡到平台期生长过程中Jun和Myc mRNA水平以及AP1、AP2和NF-kappa B的DNA结合活性。因此，这些结果表明，射频辐射不太可能在这些条件下引起该细胞系的一般应激反应，然而，在过渡到平台期的指数型细胞和暴露于835.62 MHz FMCW微波的平台期细胞中，For基因的表达水平显著增加(类似于2倍，P=0.001)。这种FOR表达的增加表明，特定基因的表达可能会受到射频暴露的影响，(C)1999年，辐射研究学会。
This study was designed to determine whether tao differently modulated radiofrequencies of the type generally used in cellular phone communications could elicit a general stress response in a biological system. The two modulations and frequencies studied were a frequently-modulated continuous wave (FMCW) with a carrier frequency of 835.62 MHz and a code division multiple-access (CDMA) modulation centered on 847.74 MHz. Changes in proto-oncogene expression, determined by measuring For, Jun, and Myc mRNA levels as well as by the DNA-binding activity of the AP1, AP2 and NF-kappa B transcription factors, were used as indicators of a general stress response, The effect of radiofrequency exposure on proto-oncogene expression was assessed (1) in exponentially growing C3H 10T 1/2 mouse embryo fibroblasts during their transition to plateau phase and (2) during transition of serum-deprived cells to the proliferation cycle after serum stimulation. Exposure of serum-deprived cells to 835.62 MHz FMCW or 847.74 MHz CDMA microwaves (at an average specific absorption rate, SAR, of 0.6 W/kg) did not significantly change the kinetics of proto-oncogene expression after serum stimulation, Similarly, these exposures did not affect either the Jun and Myc mRNA levels or the DNA-binding activity of AP1, AP2 and NF-kappa B in exponential cells during transit to plateau-phase growth. Therefore, these results suggest that the radiofrequency exposure is unlikely to elicit a general stress response in cells of this cell, line under these conditions, However, statistically significant increases (similar to 2-fold, P = 0.001) in For mRNA levels were detected in exponential cells in transit to the plateau phase and in plateau-phase cells exposed to 835.62 MHz FMCW microwaves, For 847.74 MHz CDMA exposure, the increase was 1.4-fold (P = 0.04). This increase in For expression suggests that expression of specific genes could be affected by radiofrequency exposure, (C) 1999 by Radiation Research Society.