Liquid Chromatography-Tandem Mass Spectrometry-Based Plasma Metabonomics Delineate the Effect of Metabolites' Stability on Reliability of Potential Biomarkers
Liquid Chromatography-Tandem Mass Spectrometry-Based Plasma Metabonomics Delineate the Effect of Metabolites' Stability on Reliability of Potential Biomarkers
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DOI：
10.1021/ac303576b
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发表时间：
2013-03-05
影响因子：
7.4
通讯作者：
Abliz, Zeper
中科院分区：
文献类型：
--
作者：
Yang, Wei;Chen, Yanhua;Abliz, Zeper
Metabonomics is an important platform for investigating the metabolites of integrated living systems and their dynamic responses to changes caused by both endogenous and exogenous factors. A metabonomics strategy based on liquid chromatography mass spectrometry/mass spectrometry in both positive and negative ion modes was applied to investigate the short-term and long-term stability of metabolites in plasma. Principal components analysis and ten types of identified metabolites were used to summarize the time-dependent change rules in metabolites systematically at different temperatures. The long-term stability of metabolites in plasma specimens stored at -80 degrees C for five years was also studied. Analysis of these subjects identified 36 metabolites with statistically significant changes in expression (p < 0.05) and found a kind of metabolite with a hundred-fold change. The stability of metabolites in blood at 4 degrees C for 24 h was also investigated. These studies show that a thorough understanding of the effects of metabolite stability are necessary for improving the reliability of potential biomarkers.
