Involvement of Can f 5 in a Case of Human Seminal Plasma Allergy
Involvement of Can f 5 in a Case of Human Seminal Plasma Allergy
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DOI：
10.1159/000336388
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Labrador-Horrillo, Moises
中科院分区：
文献类型：
--
作者：
Basagana, Maria;Bartolome, Borja;Labrador-Horrillo, Moises
背景资料：存在IgE与狗皮屑提取物结合，而不存在针对所述狗过敏原（Can f 1、2、3和4）的IgE抗体，这意味着存在其他尚待鉴定的狗过敏原。最近，从狗尿中分离出一种IgE结合蛋白，并鉴定为前列腺激肽释放酶;它已被命名为Can f 5。已经描述了狗皮屑过敏原和人前列腺特异性抗原（PSA）之间的交叉反应性。本研究的目的是确定狗皮屑过敏原与PSA的交叉反应，并证明其在我们的患者与人类精浆过敏的临床意义。方法：采用SDS-PAGE免疫印迹法和抑制试验。进行质谱分析以鉴定参与过敏反应的蛋白质。结果如下：用狗前列腺分泌物中的IgE结合蛋白进行SDS-PAGE免疫印迹抑制，显示总IgE结合抑制28 kDa的IgE反应条带，鉴定为PSA。从狗前列腺液中电洗脱的蛋白质经质谱鉴定为Can f5。与患者血清孵育的人精浆IgE免疫印迹显示两个IgE结合带（28和32.7 kDa）。这两个SDS-PAGE免疫印迹抑制试验，与人精浆或纯化的PSA在固相中，表现出完全的IgE结合抑制时，过敏性患者的血清与狗皮屑提取物或重组Can f 5预孵育。结论：狗皮屑过敏原，显示与人PSA的交叉反应性已被确定，原来是最近描述的Can f 5。我们证明了这种交叉反应性在患者中的临床相关性。版权所有（C）2012 S. Karger AG，巴塞尔
Background: The existence of IgE binding to dog dander extract without IgE antibodies against the described dog allergens (Can f 1, 2, 3 and 4) implies the presence of other dog allergens yet to be identified. Recently, an IgE-binding protein was isolated from dog urine and identified as prostatic kallikrein; it has been named Can f 5. Cross-reactivity between a dog dander allergen and human prostate-specific antigen (PSA) has been described. The aim of this study was to identify the dog dander allergen that presents cross-reactivity with PSA and demonstrate its clinical relevance in our patient with human seminal plasma allergy. Methods: SDS-PAGE immunoblotting and inhibition tests were performed. Mass spectrometry was carried out to identify the protein involved in the allergy reactions. Results: SDS-PAGE immunoblotting-inhibition with an IgE-binding protein from dog prostatic secretion showed total IgE binding inhibition to a 28-kDa IgE-reactive band identified as PSA. The electroeluted protein from dog prostatic secretion was identified by mass spectrometry as Can f 5. IgE immunoblotting of human seminal plasma incubated with the serum of the patient revealed two IgE-binding bands (28 and 32.7 kDa). Both SDS-PAGE immunoblotting inhibition assays, with human seminal plasma or purified PSA in solid phase, showed complete IgE binding inhibition when the serum of the anaphylactic patient was pre-incubated with dog dander extract or recombinant Can f 5. Conclusions: The dog dander allergen that shows cross-reactivity with human PSA has been characterized and turns out to be the recently described Can f 5. We demonstrated the clinical relevance of this cross-reactivity in a patient. Copyright (C) 2012 S. Karger AG, Basel