Electrochemical biosensing of methyl parathion pesticide based on acetylcholinesterase immobilized onto Au-polypyrrole interlaced network-like nanocomposite
Electrochemical biosensing of methyl parathion pesticide based on acetylcholinesterase immobilized onto Au-polypyrrole interlaced network-like nanocomposite
复制标题
基于金-聚吡咯交错网络状纳米复合材料固定乙酰胆碱酯酶的甲基对硫磷农药的电化学生物传感
DOI：
10.1016/j.bios.2008.11.012
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发表时间：
2009-03-15
影响因子：
12.6
通讯作者：
Zhang, Lizhi
中科院分区：
文献类型：
--
作者：
Gong, Jingming;Wang, Lianyi;Zhang, Lizhi
我们开发了一种基于乙酰胆碱酯酶(AChE)固定在金纳米粒子-聚吡咯纳米线复合膜修饰玻碳电极(标记为AChE-Au-PPy/GCE)上的高灵敏有机磷农药生物传感器的设计方法。其中，生成的Au纳米颗粒(AuNPs)均匀分布在交错的PPy纳米线(PPy NWS)基质上，构建了一个三维的多孔网络。这种网络状的纳米复合材料不仅为保持AChE的生物活性提供了一个生物相容的微环境，而且在改善OPS的传感性能方面表现出了很强的协同效应。AuNPs和PPyNWs的结合极大地催化了酶促生成的硫代胆碱产物的氧化，从而提高了检测灵敏度。根据有机磷农药对乙酰胆碱酯酶活性的抑制作用，以甲基对硫磷为模型OP化合物，优化了有机磷农药检测条件。甲基对硫磷的抑制作用与其浓度在0.005.5~0.12和0.5~4.5mgmL-1范围内呈良好的线性关系。方法检出限为2ngmL(-1)。所研制的生物传感器具有良好的重复性和较好的稳定性。本研究为有机磷农药的分析提供了一种新的有希望的工具。(C)2008爱思唯尔B.V.保留所有权利。
We developed a simple strategy for designing a highly sensitive electrochemical biosensor for organophosphate pesticides (OPs) based on acetylcholinesterase (AChE) immobilized onto Au nanoparticles-polypyrrole nanowires composite film modifid glassy carbon electrode (labeled as AChE-Au-PPy/GCE). Where, the generated Au nanoparticles (AuNPs) were homogenously distributed onto the interlaced PPy nanowires (PPy NWs) matrix, constructing a three-dimensional porous network. This network-like nanocomposite not only provided a biocompatible microenvironment to keep the bioactivity of AChE, but also exhibited a strong synergetic effect on improving the sensing properties of OPs. The combination of AuNPs and PPyNWs greatly catalyzed the oxidation of the enzymatically generated thiocholine product, thus increasing the detection sensitivity. On the basis of the inhibition of OPs on the enzymatic activity of AChE, the conditions for OPs detection were optimized by using methyl parathion as a model OP compound. The inhibition of methyl parathion was proportional to its concentration ranging from 0.005 to 0.12 and 0.5 to 4.5 mu g mL(-1). The detection limit was 2 ng mL(-1). The developed biosensor exhibited good reproducibility and acceptable stability. This study provides a new promise tool for analysis of organophosphate pesticides. (C) 2008 Elsevier B.V. All rights reserved.