Facilitated assignment of adenine H2 resonances in oligonucleotides using homonuclear long-range couplings.
Facilitated assignment of adenine H2 resonances in oligonucleotides using homonuclear long-range couplings.
复制标题
使用同核长程耦合促进寡核苷酸中腺嘌呤 H2 共振的分配。
DOI：
10.1021/ja900771b
复制
发表时间：
2009
影响因子：
15
通讯作者：
Germann,MarkusW
中科院分区：
文献类型：
--
作者：
Zhang,Jin;Spring,Alexander;Germann,MarkusW
测定了腺嘌呤H_2和H_8质子间的小的同型偶联常数（0.5 ± 0.2Hz），并用于DNA寡核苷酸中腺嘌呤残基H_2质子的归属。这种方法的实用性证明了两个不同的DNA序列中的D2O，我们显示了一个快速和明确的分配的A H2共振。
The small homonuclear coupling constant between H2 and H8 protons of adenine (0.5 ± 0.2 Hz) was determined and exploited to assign the H2 proton of adenine residues in DNA oligonucleotides. The utility of this approach is demonstrated for two different DNA sequences in D2O, for which we show a rapid and unambiguous assignment of the A H2 resonances.