Search for substrates for prolyl oligopeptidase in porcine brain
Search for substrates for prolyl oligopeptidase in porcine brain
复制标题
DOI：
10.1016/j.peptides.2005.05.016
复制
发表时间：
2005-12-01
期刊：
影响因子：
3
通讯作者：
Lambeir, AM
中科院分区：
文献类型：
--
作者：
Brandt, I;De Vriendt, K;Lambeir, AM
脯氨酰寡肽酶（PO）的功能与中枢神经系统的几种疾病有关。本研究的目的是鉴定重组猪PO在猪脑中的内源性底物。从总脑匀浆中提取较小的多肽，并在与PO孵育之前通过二维色谱法进行分级。对照品和孵育样品（标记为潜在底物）之间的质谱变化。使用MSMS肽测序技术，我们确定了几个片段的细胞内蛋白作为潜在的底物，这打开了新的视角，发现PO在细胞内空间的功能。(c)2005年爱思唯尔公司All rights reserved.
The function of prolyl oligopeptidase (PO) has been associated with several disorders of the central nervous system. The purpose of this study was to identify endogenous substrates for recombinant porcine PO in porcine brain. The smaller polypeptides were extracted from total brain homogenates and fractionated by two-dimensional chromatography prior to incubation with PO. Shifts in the mass spectrum between the control and the incubated sample, marked potential substrates. Using MSMS peptide sequencing techniques, we identified several fragments of intracellular proteins as potential substrates, which opens new perspectives for finding the function of PO in the intracellular space. (c) 2005 Elsevier Inc. All rights reserved.