Genome-Wide Analysis of RNA-Protein Interactions in Plasmodium falciparum Using eCLIP-Seq.
Genome-Wide Analysis of RNA-Protein Interactions in Plasmodium falciparum Using eCLIP-Seq.
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DOI：
10.1007/978-1-0716-1681-9_9
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发表时间：
2021-01-01
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影响因子：
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通讯作者：
Le Roch, Karine G
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文献类型：
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作者：
Hollin, Thomas;Abel, Steven;Le Roch, Karine G
在过去的几十年里，RNA-蛋白质复合物及其结合位点的鉴定具有挑战性。最近，基于交联、免疫沉淀和高通量测序的技术得到了发展。一种称为 eCLIP-seq 的优化方法能够以核苷酸分辨率精确识别目标 RNA 以及转录组范围内的结合位点。在这里，我们描述了人类疟原虫恶性疟原虫无性阶段的 eCLIP-seq 方案。该方法可以促进该生物体中 RNA 结合蛋白的表征，但目前可用的数据很少。
Over the last decades, identification of RNA-proteins complexes and their binding sites was challenging. Recently, techniques based on crosslinking, immunoprecipitation, and high-throughput sequencing have been developed. An optimized method, called eCLIP-seq, enables to identify precisely the targeted RNAs as well as the transcriptome-wide binding sites at nucleotide resolution. Here we describe the eCLIP-seq protocol in asexual stages of the human malaria parasite, Plasmodium falciparum. This method could facilitate the characterization of RNA-binding proteins in this organism for which few data are currently available.