Intranasal salvinorin A improves neurological outcome in rhesus monkey ischemic stroke model using autologous blood clot
Intranasal salvinorin A improves neurological outcome in rhesus monkey ischemic stroke model using autologous blood clot
复制标题
鼻内Salvinorin A使用自体血凝块改善恒河猴缺血性中风模型的神经系统结果
DOI：
10.1177/0271678x20938137
复制
发表时间：
2020-07-02
影响因子：
6.3
通讯作者：
Ji, Xunming
中科院分区：
文献类型：
--
作者：
Wu, Longfei;Wu, Di;Ji, Xunming
Salvinorin A （SA）在啮齿动物缺血性卒中模型中发挥神经保护作用并改善神经预后。在这项研究中，我们研究了鼻内给药SA是否可以改善猴子缺血性卒中模型的神经预后。采用自体血凝块阻断成年雄性恒河猴大脑中动脉M2段，建立脑卒中模型。8只成年恒河猴缺血20 min后随机给予SA或10%二甲亚砜作为对照。采用核磁共振成像技术确定缺血程度和损伤程度。在28天的观察期内，使用非人类灵长类动物中风量表（NHPSS）评估神经功能。与对照组相比，SA显著降低了梗死面积（3.9±0.7 cm3 vs. 7.2±1.0 cm3, P = 0.002）、占位效应（0.3±0.2% vs. 1.4±0.3%,P = 0.002）和病灶内弥散限制（- 28.2±11.0% vs. - 51.5±7.1%,P = 0.012）。在28天的观察期内，与对照组相比，SA显著降低了NHPSS评分，接近正常（P = 0.005）。使用自体血凝块的恒河猴缺血性脑卒中模型中，鼻内SA可减少梗死面积并改善神经预后。
Salvinorin A (SA) exerts neuroprotection and improves neurological outcomes in ischemic stroke models in rodents. In this study, we investigated whether intranasal SA administration could improve neurological outcomes in a monkey ischemic stroke model. The stroke model was induced in adult male rhesus monkeys by occluding the middle cerebral artery M2 segment with an autologous blood clot. Eight adult rhesus monkeys were randomly administered SA or 10% dimethyl sulfoxide as control 20 min after ischemia. Magnetic resonance imaging was used to confirm the ischemia and extent of injury. Neurological function was evaluated using the Non-Human Primate Stroke Scale (NHPSS) over a 28-day observation period. SA significantly reduced infarct volume (3.9 ± 0.7 cm3 vs. 7.2 ± 1.0 cm3; P = 0.002), occupying effect (0.3 ± 0.2% vs. 1.4 ± 0.3%; P = 0.002), and diffusion limitation in the lesion (−28.2 ± 11.0% vs. −51.5 ± 7.1%; P = 0.012) when compared to the control group. SA significantly reduced the NHPSS scores to almost normal in a 28-day observation period as compared to the control group (P = 0.005). Intranasal SA reduces infarct volume and improves neurological outcomes in a rhesus monkey ischemic stroke model using autologous blood clot.