Pattern recognition receptors and differentiation antigens define murine myeloid cell heterogeneity ex vivo
Pattern recognition receptors and differentiation antigens define murine myeloid cell heterogeneity ex vivo
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DOI：
10.1002/eji.200324003
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发表时间：
2003-08-01
影响因子：
5.4
通讯作者：
Gordon, S
中科院分区：
文献类型：
--
作者：
Taylor, PR;Brown, GD;Gordon, S
炎症反应中髓系细胞的募集在炎症的传播和消退中起着重要作用。然而，这些细胞在小鼠体内的鉴定和特征一直受到功能和表型水平上的细胞异质性的阻碍。我们使用新的和已建立的髓系标记物，定义了流式细胞术快速鉴定小鼠组织中单核细胞(M-o)、巨噬细胞(Mphi)、中性粒细胞(N-EU)和嗜酸性粒细胞(E-os)的标准。这些标准被应用于对幼稚小鼠和实验诱导的局部和全身炎症的小鼠的研究，也应用于肿瘤进展的小鼠模型。我们发现，小鼠7/4抗原和β-葡聚糖受体Dectin-1对于将髓系细胞细分为单个群体特别有用，即使当炎症条件调节它们的表面表达时也是如此。此外，7/4的表达可以区分最近招募到某个地点的M-O细胞和已经存在的常驻细胞。这些研究突出了小鼠M-o/Mphi-谱系的异质性，定义了小鼠髓系细胞的扩展表型，并极大地促进了这些细胞在非常不同的炎症模型中的体外表征。
Recruitment of myeloid cells during inflammatory reactions plays an important role in the propagation and resolution of inflammation. However, the identification and characterization of these cells in mice has been hampered by cellular heterogeneity at the functional and phenotypic level. We have defined criteria for the rapid flow-cytometric identification of monocytes (M-o), macrophages (Mphi), neutrophils (N-eu) and eosinophils (E-os) in murine tissues using novel and established myeloid markers. These criteria were applied to the study of naive mice and mice with experimentally induced inflammation, both local and systemic, and also to a murine model of tumor progression. We show that the murine 7/4 antigen and the beta-glucan receptor, Dectin-1, are particularly useful for the sub-division of myeloid cells into individual populations, even when inflammatory conditions modulate their surface expression. Furthermore, 7/4 expression allows distinction between M-o recently recruited to a site and the resident cells already present. These studies highlight the heterogeneity of the murine M-o/Mphi-lineage, define an extended phenotype for murine myeloid cells and greatly facilitate the ex vivo characterization of these cells during very different models of inflammation.