Label-free photoacoustic microscopy of peripheral nerves
Label-free photoacoustic microscopy of peripheral nerves
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DOI：
10.1117/1.jbo.19.1.016004
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发表时间：
2014-01-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Matthews, Thomas Paul;Zhang, Chi;Wang, Lihong V.
周围神经病变是一种常见的神经系统疾病，影响着全世界数百万人。这种情况的诊断和治疗往往受到阻碍，在客观的，非侵入性的测量神经纤维的困难。光声显微镜（PAM）能够在没有外源性对比剂的情况下获得高分辨率的周围神经特异性图像。我们展示了第一个使用PAM的周围神经概念验证成像。正如标准组织学和光声光谱学所证实的，光声信号的来源是髓鞘，神经中脂质的主要来源。夹在两层鸡组织之间的提取的坐骨神经通过PAM成像以模拟体内情况。神经内可见由髓鞘包裹的轴突束形成的有序纤维结构。随着进一步的技术改进，PAM可能被应用于监测和诊断周围神经病变。(C)2014年，美国光电仪器工程师学会（SPIE）
Peripheral neuropathy is a common neurological problem that affects millions of people worldwide. Diagnosis and treatment of this condition are often hindered by the difficulties in making objective, noninvasive measurements of nerve fibers. Photoacoustic microscopy (PAM) has the ability to obtain high resolution, specific images of peripheral nerves without exogenous contrast. We demonstrated the first proof-of-concept imaging of peripheral nerves using PAM. As validated by both standard histology and photoacoustic spectroscopy, the origin of photoacoustic signals is myelin, the primary source of lipids in the nerves. An extracted sciatic nerve sandwiched between two layers of chicken tissue was imaged by PAM to mimic the in vivo case. Ordered fibrous structures inside the nerve, caused by the bundles of myelin-coated axons, could be observed clearly. With further technical improvements, PAM can potentially be applied to monitor and diagnose peripheral neuropathies. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)