SseF, a type III effector protein from the mammalian pathogen Salmonella enterica, requires resistance-gene-mediated signalling to activate cell death in the model plant Nicotiana benthamiana.
SseF, a type III effector protein from the mammalian pathogen Salmonella enterica, requires resistance-gene-mediated signalling to activate cell death in the model plant Nicotiana benthamiana.
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DOI：
10.1111/j.1469-8137.2012.04124.x
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发表时间：
2012-06
期刊：
影响因子：
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通讯作者：
Suayib Ustün;P. Müller;R. Palmisano;M. Hensel;F. Börnke
中科院分区：
文献类型：
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作者：
Suayib Ustün;P. Müller;R. Palmisano;M. Hensel;F. Börnke
许多革兰氏阴性病原菌的III型效应蛋白（T3 Es）操纵高度保守的细胞过程，表明在不同进化起源的宿主感染期间毒力机制的保守性。为了确定保守的效应子功能，我们使用了一种跨王国的方法，其中我们表达了选择的T3 E从哺乳动物病原体沙门氏菌enterica在烟草本氏叶，并寻找可能的毒力或无毒力表型。我们证明了S. enterica在N.通过根癌农杆菌渗透或当由野油菜黄单胞菌水疱致病变种（Xcv）通过III型分泌系统递送时，本塞姆氏菌可将本塞姆氏菌转化为本塞姆氏菌。SseF引起HR样症状的能力在skp 1的G2等位基因（SGT 1）的抑制基因沉默后丧失，表明S. enterica T3 E可能被N.本萨米亚纳Xcv易位AvrRpt 2-SseF融合蛋白限制了其在烟草叶片内的增殖。本萨米亚纳细菌的生长没有受到损害，但症状的发展，而加速在一个兼容的相互作用与敏感的辣椒（辣椒）植物。我们认为S. enterica T3 E SseF可能被N. benthamiana，导致效应触发免疫。
Type III effector proteins (T3Es) of many Gram-negative pathogenic bacteria manipulate highly conserved cellular processes, indicating conservation in virulence mechanisms during the infection of hosts of divergent evolutionary origin. In order to identify conserved effector functions, we used a cross-kingdom approach in which we expressed selected T3Es from the mammalian pathogen Salmonella enterica in leaves of Nicotiana benthamiana and searched for possible virulence or avirulence phenotypes. We show that the T3E SseF of S. enterica triggers hypersensitive response (HR)-like symptoms, a hallmark of effector-triggered immunity in plants, either when transiently expressed in leaves of N. benthamiana by Agrobacterium tumefaciens infiltration or when delivered by Xanthomonas campestris pv vesicatoria (Xcv) through the type III secretion system. The ability of SseF to elicit HR-like symptoms was lost upon silencing of suppressor of G2 allele of skp1 (SGT1), indicating that the S. enterica T3E is probably recognized by an R protein in N. benthamiana. Xcv translocating an AvrRpt2-SseF fusion protein was restricted in multiplication within leaves of N. benthamiana. Bacterial growth was not impaired but symptom development was rather accelerated in a compatible interaction with susceptible pepper (Capsicum annuum) plants. We conclude that the S. enterica T3E SseF is probably recognized by the plant immune system in N. benthamiana, resulting in effector-triggered immunity.