Saccharophagus degradans gen. nov., sp nov., a versatile marine degrader of complex polysaccharides
Saccharophagus degradans gen. nov., sp nov., a versatile marine degrader of complex polysaccharides
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DOI：
10.1099/ijs.0.63627-0
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发表时间：
2005-07-01
影响因子：
2.8
通讯作者：
Weiner, RM
中科院分区：
文献类型：
--
作者：
Ekborg, NA;Gonzalez, JM;Weiner, RM
γ-变形菌属于微球菌属，是一组新兴的复杂的碳水化合物降解海洋细菌。以前，所有的代表都被放置在Microbulbifer内或未分类。最近，一个新的属，Teredinibacter，由一个单一的物种，Teredinibacter turnerae代表，形成包括这些生物体的内共生分支。在这项研究中，基于16 S rRNA基因序列相似性和表型分析，提出了一个新的属，食蟹猴，以容纳最多功能的海洋碳水化合物降解菌，食蟹猴degradans gen. nov.，新种2-40（T）（=ATCC 43961（T）=DSM 17024（T））。S.降解菌2-40（T）能降解琼脂、海藻酸钠、几丁质、纤维素、褐藻糖胶、海带多糖、果胶、支链淀粉、淀粉和木聚糖等10种复合多糖。S. degradans 2-40（T）与Microbulbiferhydrolyticus IRE-31（T）的16 SrRNA基因序列相似性为90.5%，与T. turneraeT 7902（T），并通过16 SrRNA基因聚类分析，进一步将其与这两个属的成员区分开来，其能够利用10种不同的复合多糖作为唯一碳源，G+C含量显著较低，脂肪酸含量存在差异。复杂的多糖降解，海洋细菌的三个属现在包括20个菌株从不同的海洋生态位。
Gammaproteobacteria belonging and related to the genus Microbulbifer are an emerging group of complex carbohydrate-degrading marine bacteria. Previously, all of the representatives were placed within Microbulbifer or were unclassified. Recently, a new genus, Teredinibacter, represented by a single species, Teredinibacter turnerae, was formed to include an endosymbiotic branch of these organisms. In this study, based on 16S rRNA gene sequence similarity and phenotypic analyses, a new genus, Saccharophagus, is proposed to accommodate the most versatile marine carbohydrate degrader yet identified, Saccharophagus degradans gen. nov., sp. nov. 2-40(T) (=ATCC 43961(T)=DSM 17024(T)). S. degradans strain 2-40(T) can degrade 10 tested complex polysaccharides: agar, alginate, chitin, cellulose, fucoidan, laminarin, pectin, pullulan, starch and xylan. S. degradans 2-40(T) shares 90.5% 16S rRNA gene sequence similarity with the type strain of the Microbulbifer type species, Microbulbifer hydrolyticus IRE-31(T), and 91.5% with T. turnerae T7902(T), and can be further distinguished from members of these two genera by 16S rRNA gene cluster analysis, the ability to utilize 10 different complex polysaccharides; as sole carbon sources, a significantly lower G+C content and differences in fatty acid content. The three genera of complex polysaccharide-degrading, marine bacteria now encompass 20 strains from diverse marine niches.