Nucleotide sequence of cucumber-mosaic-virus RNA 2 reveals a translation product significantly homologous to corresponding proteins of other viruses.
Nucleotide sequence of cucumber-mosaic-virus RNA 2 reveals a translation product significantly homologous to corresponding proteins of other viruses.
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黄瓜花叶病毒RNA 2的核苷酸序列揭示了与其他病毒的相应蛋白质显着同源的翻译产物。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
Robert H. Symons
中科院分区：
文献类型：
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作者：
M. Rezaian;Rhys H. V. Williams;K. H. Gordon;Allan R. Gould;Robert H. Symons
对黄瓜花叶病毒(CMV)Q株系RNA2的3035个氨基酸残基(Mr 1.03×10(6))的核苷酸序列进行了测序和双脱氧测序。一个长的开放阅读框从RNA2的5‘端的第二个8月开始，编码839个氨基酸(Mr 94333)。该框架的侧翼区域在5‘端有92个核苷酸，在3’端有423个核苷酸。计算机序列分析表明，CMV RNA2与雀麦花叶病毒(BMV)和紫花叶病毒(AMV)的RNA2有显著的同源性，也与烟草花叶病毒(TMV)的RNA编码183 kDa蛋白的通读部分有显著的同源性。CMV RNA2翻译产物中央约400个氨基酸与BMV和AMV编码的相应蛋白以及TMV 183-kDa蛋白的通读部分具有显著的同源性。CMV和BMV RNA翻译产物的疏水性图谱也有明显的相似性。因此，CMV与BMV、AMV和TMV的进化分化程度依次为：CMV>AMV>TMV。
The nucleotide sequence of the 3035 residues of RNA 2 (Mr 1.03 X 10(6) ) of the Q strain of cucumber mosaic virus (CMV) was determined by sequencing M13 clones of the RNA 2 cDNA and by dideoxy sequencing using primers prepared either from M13 clones or by chemical synthesis. A single long open reading frame starts at the second AUG from the 5' end of RNA 2 and encodes 839 amino acids (Mr 94333). This frame has flanking regions of 92 nucleotides at the 5' terminus and 423 nucleotides at the 3' terminus. Computer analysis of the nucleotide sequence showed that CMV RNA 2 has a significant homology with RNA 2 of brome mosaic virus (BMV) and alfalfa mosaic virus (AMV) and also with a region for tobacco mosaic virus (TMV) RNA encoding the read-through part of the 183-kDa protein. About 400 amino acids in the central region of the CMV RNA 2 translation product have a striking homology with the corresponding proteins encoded by BMV and AMV and with the read-through part of the TMV 183-kDa protein. Hydrophobicity plots of CMV and BMV RNA translation products also had apparent similarities. It is concluded that CMV is related to BMV, AMV and TMV in order of increasing evolutionary divergence.
影响因子：
5.6
作者：
AHLQUIST, P;DASGUPTA, R;KAESBERG, P
通讯作者：
KAESBERG, P