Experimental study on “ischemic memory” of myocardium with different ischemic degrees by 18F‑FDG PET/CT
Experimental study on “ischemic memory” of myocardium with different ischemic degrees by 18F‑FDG PET/CT
复制标题
18F-FDG PET/CT对不同缺血程度心肌“缺血记忆”的实验研究
DOI：
10.1007/s12149-019-01411-3
复制
发表时间：
2019
影响因子：
2.6
通讯作者：
Xuemei Wang
中科院分区：
文献类型：
--
作者：
Jianbo Li;Na Zheng;Guojian Zhang;Baoliang Bao;Lei Liu;Linjun Hao;Xuemei Wang
方法结扎大鼠冠状动脉左前降支建立缺血再灌注模型，采用18F-FDG PET/CT技术，比较禁食和进食状态下大鼠心肌糖代谢及缺血心肌细胞范围的变化，探讨心肌再灌注后糖代谢的变化及进食状态下心肌“缺血记忆”的时间窗。禁食实验中，长期缺血大鼠（n= 10）在禁食或进食状态下，于造模后24 h进行18 F-FDG PET/CT检查。通过计算感兴趣体积（VOI）和平均标准摄取值（SUV mean）比较缺血心肌范围和糖代谢。在喂养状态下，短、中、长缺血组大鼠分别于24、48、72 h行18F-FDG PET/CT扫描，长缺血组大鼠于96 h行18F-FDG PET/CT扫描，计算VOI和SUV mean，并进行比较。而在喂养状态下，同一区域心肌出现“局灶性“18F-FDG摄取缺损，差异无统计学意义（P> 0.05）。(2)在喂养状态下，3组大鼠24 h和48 h心肌PET显像均显示左室壁心尖部有18F-FDG摄取缺损区。72 h时，短、中缺血组未见异常18F-FDG摄取缺损区，而长缺血组18F-FDG摄取缺损区于96 h时消失。对3组数据进行重复测量方差分析，心肌缺血程度与“缺血记忆”时间窗、心肌缺损体积差之间差异均有统计学意义（P <0. 05）。在饲养条件下，短、中心肌缺血的“缺血记忆”时间窗至少为48 h，长心肌缺血的“缺血记忆”时间窗至少为72 h。本研究提示，随着心肌缺血程度的加重，“缺血记忆”时间窗也随之延长。
ObjectiveGlucose metabolism and ranges of ischemic cardiomyocytes in model rats under fasting or feeding were compared by18F-FDG PET/CT, respectively, to investigate the changes of glucose metabolism after myocardial reperfusion, and to determine time window of myocardial “ischemic memory” under feeding.MethodsThe ischemic–reperfusion model rats were established by ligating the left anterior descending coronary artery of rats. In fasting–feeding experiment, long-ischemia rats (n= 10) under fasting or feeding were subjected to18F-FDG PET/CT at 24 h after modeling. Ischemic myocardium range and glucose metabolism were compared by calculating volume of interest (VOI), and mean standard uptake value (SUVmean). Under feeding, model rats in short, intermediate and long-ischemia groups were subjected to18F-FDG PET/CT at 24, 48, and 72 h and long-ischemia rats were also subjected to18F-FDG PET/CT at 96 h, and VOI and SUVmean were calculated and compared.Results(1) Under fasting, myocardial ischemic area of model rats showed “focal”18F-FDG uptake, while “focal”18F-FDG uptake defect appeared in the same area of myocardium under feeding and the difference was not statistically significant (P> 0.05). (2) Under feeding, PET myocardial images of model rats in three groups at 24 h and 48 h showed that there was an18F-FDG uptake defect area near the apex of left ventricular wall. The images at 72 h showed that there was no abnormal18F-FDG uptake defect area in short and intermediate-ischemia groups, while18F-FDG uptake defect area in long-ischemia group disappeared at 96 h. Variance analysis of repeated measures was performed for data of three groups, which showed there was statistical significance between myocardial ischemia degree and “ischemic memory” time window (P< 0.05), and also between myocardial ischemia degree and difference of myocardial defect volume (P< 0.05).ConclusionsUnder feeding, recent myocardial ischemia could be diagnosed by18F-FDG PET/CT. Under feeding, “ischemic memory” time window for short and intermediate-myocardial ischemia was at least 48 h and for long ischemia was at least 72 h. This study suggested that as the degree of myocardial ischemia increased, “ischemic memory” time window also extended.