Gene expression profiling in Salmonella Choleraesuis-infected porcine lung using a long oligonucleotide microarray
Gene expression profiling in Salmonella Choleraesuis-infected porcine lung using a long oligonucleotide microarray
复制标题
DOI：
10.1007/s00335-005-0155-3
复制
发表时间：
2006-07-01
期刊：
影响因子：
2.5
通讯作者：
Tuggle, Christopher K.
中科院分区：
文献类型：
--
作者：
Zhao, Shu-Hong;Kuhar, Daniel;Tuggle, Christopher K.
了解食用动物对病原菌感染的转录反应具有重要的基础和应用价值。为了确定猪霍乱沙门氏菌(SC)感染后的转录反应，用13,297个寡核苷酸芯片分析了来自对照、接种后24小时(HPI)和感染SC的48-HPI猪肺组织的RNA。总共有57个基因显示出差异表达(p<；0.001；错误发现率=12%)。61个基因的实时定量聚合酶链式反应(qRT-PCR)被用来验证微阵列的结果，并确定感染反应的途径。在芯片分析鉴定的33个差异表达基因中，有23个基因被qRT-PCR结果证实。一个新的发现是，两个转谷氨酰胺酶家族基因(TGM1和TGM3)在接种后表达显著增加；它们与其他几个凋亡基因相结合，表明在SC感染过程中诱导了凋亡途径。在感染期间发生了主要的T辅助1型免疫反应，干扰素-γ(IFNG)在48HPI时显著增加。在猪肺部感染过程中，干扰素诱导的基因(GBP1、GBP2、C1S、C1R、MHC2TA、PSMB8、TAP1、TAP2)表达增强。这些数据代表了对控制一种重要的猪呼吸道和食源性细菌感染的基因调控途径的首次彻底调查。
Understanding the transcriptional response to pathogenic bacterial infection within food animals is of fundamental and applied interest. To determine the transcriptional response to Salmonella enterica serovar Choleraesuis (SC) infection, a 13,297-oligonucleotide swine array was used to analyze RNA from control, 24-h postinoculation (hpi), and 48-hpi porcine lung tissue from pigs infected with SC. In total, 57 genes showed differential expression (p < 0.001; false discovery rate = 12%). Quantitative real-time PCR (qRT-PCR) of 61 genes was used to confirm the microarray results and to identify pathways responding to infection. Of the 33 genes identified by microarray analysis as differentially expressed, 23 were confirmed by qRT-PCR results. A novel finding was that two transglutaminase family genes (TGM1 and TGM3) showed dramatic increases in expression postinoculation; combined with several other apoptotic genes, they indicated the induction of apoptotic pathways during SC infection. A predominant T helper 1-type immune response occurred during infection, with interferon gamma (IFNG) significantly increased at 48 hpi. Genes induced by IFNs (GBP1, GBP2, C1S, C1R, MHC2TA, PSMB8, TAP1, TAP2) showed increased expression during porcine lung infection. These data represent the first thorough investigation of gene regulation pathways that control an important porcine respiratory and foodborne bacterial infection.