Genetic and molecular characterization of uveal melanoma cell lines.
Genetic and molecular characterization of uveal melanoma cell lines.
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DOI：
10.1111/j.1755-148x.2012.00971.x
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发表时间：
2012-03
影响因子：
4.3
通讯作者：
Woodman SE
中科院分区：
文献类型：
--
作者：
Griewank KG;Yu X;Khalili J;Sozen MM;Stempke-Hale K;Bernatchez C;Wardell S;Bastian BC;Woodman SE
最近鉴定的葡萄膜黑色素瘤中GNAQ或GNA11的频繁激活突变提供了一个机会，以更好地了解这种黑色素瘤亚型的发病机制，并开发合理的治疗方法来靶向这些突变介导的细胞效应。来自葡萄膜黑色素瘤肿瘤的细胞系是这些类型分析的基本工具。我们报告了相关黑色素瘤基因的突变状态，感兴趣的蛋白质的表达水平和研究界使用的一组葡萄膜黑色素瘤细胞系的DNA指纹图谱。这项研究代表了迄今为止对葡萄膜黑色素瘤细胞系进行的最全面的分子分析。数据证实了GNAQ和GNA11突变在体外的相互排斥性质。BRAF、NRAS、KIT、PI3K和AKT突变的缺乏揭示了GNAQ和GNA11葡萄膜黑色素瘤细胞在黑色素瘤类型中是不同的。所提供的数据旨在为研究者选择适当的模型系统和协助鉴定葡萄膜黑色素瘤细胞系提供参考。
The recent identification of frequent activating mutations in GNAQ or GNA11 in uveal melanoma provides an opportunity to better understand the pathogenesis of this melanoma subtype, and to develop rational therapeutics to target the cellular effects mediated by these mutations. Cell lines from uveal melanoma tumors are an essential tool for these types of analyses. We report the mutation status of relevant melanoma genes, expression levels of proteins of interest and DNA fingerprinting of a panel of uveal melanoma cell lines used in the research community. This study represents the most comprehensive molecular analysis of uveal melanoma cell lines performed to date. The data confirms the mutually exclusive nature of GNAQ and GNA11 mutations in vitro. The lack of BRAF, NRAS, KIT, PI3K, and AKT mutations reveal GNAQ and GNA11 uveal melanoma cells to be distinct among melanoma types. The data provided is intended as a reference for investigators to select appropriate model systems and assist with authentication of uveal melanoma cell lines.