Macrophage mannose receptor 1 and S100A9 were identified as serum diagnostic biomarkers for colorectal cancer through a label-free quantitative proteomic analysis
Macrophage mannose receptor 1 and S100A9 were identified as serum diagnostic biomarkers for colorectal cancer through a label-free quantitative proteomic analysis
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DOI：
10.3233/cbm-150560
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发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Gao, Chun-Fang
中科院分区：
文献类型：
--
作者：
Fan, Nai-Jun;Chen, Hong-Mei;Gao, Chun-Fang
背景：可用于早期检测的简单血液检查对于结直肠癌（CRC）的最终控制和预防至关重要。在这项研究中，我们进行了血清蛋白质组学分析的CRC和健康志愿者，以确定新的生物标志物参与CRC.METHOD：鸟枪蛋白质组学方法被应用于确定血清蛋白质中的三个CRC和三个健康志愿者的血清样本中使用相结合的高效液相色谱和质谱。进行无标记蛋白质谱分析以定量蛋白质并比较CRC和健康志愿者的谱。两个差异表达的蛋白质进一步通过western blot分析进行验证。结果：在373个蛋白质中，有69个蛋白质与大肠癌相关，其中33个蛋白质表达上调，36个蛋白质表达下调。基因本体和大卫数据库用于确定不同蛋白质的位置和功能。在69个与结直肠癌相关的蛋白质中，有2个蛋白质，即巨噬细胞甘露糖受体1（macrophage mannose receptor 1，MRC1）和S100A9，在结直肠癌组织中表达上调，并可通过ELISA方法准确鉴定结直肠癌。
BACKGROUND: Simple blood tests that could be used for early detection are crucial for the ultimate control and prevention of colorectal cancer (CRC). In this study, we performed a serum proteomic analysis of CRC and health volunteers to identify the novel biomarkers involved in CRC.METHOD: A shotgun proteomic method was applied to identify serum proteins in the serum samples of three CRC and three health volunteers using a combination of high-performance liquid chromatography and mass spectrometry. Label-free protein profiling was conducted to quantify the proteins and compare the profiles of the CRC and health volunteers. Two differentially expressed proteins were further validated by western blot analysis. Quantity analysis was performed through enzyme linked immunosorbent assay (ELISA) in serum from 96 healthy and 118 CRC volunteers.RESULTS: Among of the 373 identified proteins, 69 were linked to CRC (33 upregulated and 36 downregulated). The Gene Ontology and DAVID databases were used to identify the location and function of the different proteins. Among the 69 proteins linked to CRC, two proteins, namely, macrophage mannose receptor 1 (MRC1) and S100A9, were verified to be upregulated in CRC by western blot analysis and could be used to identify CRC from healthy volunteers with high accuracy through ELISA analysis.CONCLUSION: MRC1 and S100A9 may contribute to the determination of the mechanisms and screening involved in CRC.