Human herpesvirus 8 seroconversion in Kenyan women by enzyme-linked immunosorbent assay and immunofluorescence assay
Human herpesvirus 8 seroconversion in Kenyan women by enzyme-linked immunosorbent assay and immunofluorescence assay
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DOI：
10.1016/j.jcv.2003.08.017
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发表时间：
2004-06-01
影响因子：
8.8
通讯作者：
Morrow, RA
中科院分区：
文献类型：
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作者：
Chohan, BH;Taylor, H;Morrow, RA
背景：人类疱疹病毒8型(HHV-8)抗体检测报告的敏感性和特异性各不相同，这取决于被测试的人群和检测方法。目的：本研究的目的是比较三种检测方法：基于HHV-8病毒裂解物的酶联免疫吸附试验(EL ISA)对肯尼亚一群HHV-8阴性的女性商业性工作者检测HHV-8血清转换的能力。用免疫荧光法检测HHV-8裂解抗原(IFA-lytic)和潜伏核抗原(IFA-LANA)。研究设计：通过酶联免疫吸附试验，从商业性工作者的预期队列中确定16名妇女血清转换为HHV-8。来自这16名女性的124份登记后样本以及登记样本都通过三种检测方法进行了HHV-8抗体检测，以监测血清转换情况。结果：在16例血清明显阴转的妇女中，有8例在第一份阳性血清同时或之前IFA抗体滴度升高，而IFA未检测到初始LANA。16名妇女中有5人入境时IFA-LANA呈阳性，表明以前感染过HHV-8。三名妇女没有证据表明IFA-溶血剂或IFA-LANA的血清转换，这三名妇女中有两人在感染HIV-1时有较高的酶联免疫吸附试验反应性。结论：当考虑IFA和IFA-LANA结果时，HHV-8抗体的EL ISA结果由阴性转为阳性，在16名女性研究队列中只有一半的人血清转换。对早期抗体反应，IFA裂解法比ELISA法更敏感。在一些既没有IFA裂解抗体也没有ELISA抗体的妇女中，IFA-LANA呈阳性，这表明它可能是潜在感染的标志。通过酶联免疫吸附试验筛查、IFA裂解试验确认阳性试验和IFA-LANA排除既往感染提供了最准确的HHV-8血清转换文件，从而推定发生HHV-8感染。(C)2003爱思唯尔B.V.保留所有权利。
Background: Human herpesvirus 8 (HHV-8) antibody tests vary in reported sensitivity and specificity, depending on the population tested and the assay. Objective: The purpose of this study was to compare the ability to detect seroconversion to HHV-8 in a cohort of HHV-8 scronegative female commercial sex workers in Kenya using three tests: HHV-8 viral lysate-based enzyme-linked immunosorbent assay (ELISA). an immunofluorescence assay for HHV-8 lytic antigens (IFA-lytic) and IFA for latent nuclear antigens (IFA-LANA). Study design: By ELISA, 16 women from a prospective cohort of commercial sex workers were identified as seroconverting to HHV-8. A total of 124 post-enrollment samples from these 16 women as well as the enrollment samples were tested for HHV-8 antibodies by all three assays to monitor seroconversion. Results: Of 16 women with apparent seroconversion by ELISA, 8 had a rise in IFA-lytic titers either concomitant with or prior to the first positive ELISA sample and no initial LANA by IFA. Five of the 16 women were IFA-LANA positive at entry, indicating prior infection with HHV-8. Three women had no evidence of seroconversion by either IFA-lytic or IFA-LANA and two of these three had increased ELISA reactivity concomitant with HIV-1 infection. Conclusions: Conversion from a negative to a positive ELISA result for HHV-8 antibody indicated seroconversion in only half of the study cohort of 16 women when IFA-lytic and IFA-LANA results were considered. The IFA-lytic assay was more sensitive than ELISA for early antibody responses. The IFA-LANA was positive in some women who had neither IFA-lytic nor ELISA antibodies suggesting it may be a marker for latent infections. Presumptive identification of incident HHV-8 infection by ELISA screening followed by IFA-lytic testing to confirm the positive test and IFA-LANA to rule out prior infection provides the most accurate documentation of HHV-8 seroconversion. (C) 2003 Elsevier B.V. All rights reserved.