Thermo-responsive monolithic materials
Thermo-responsive monolithic materials
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DOI：
10.1016/j.chroma.2008.07.042
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发表时间：
2008-09-05
影响因子：
4.1
通讯作者：
Titirici, Maria-Magdalena
中科院分区：
文献类型：
--
作者：
Roohi, Farnoosh;Antonietti, Markus;Titirici, Maria-Magdalena
高效液相色谱最近的主要改进之一是引入了整体硅胶色谱柱，与普通硅胶小球相比，它具有分离速度快和背压低的优点。在这里，我们提出了一种有趣的替代这种反相整体柱的方法，即通过一种方便的将热响应性聚合物耦合到亲水性硅胶整体柱的途径。采用可逆加成断裂链转移(RAFT)聚合技术对聚N-异丙基丙烯酰胺(PNIPAM)进行溶液聚合，并将其原位偶联到氨基修饰的硅胶整体柱上。将这些色谱柱与RP-18整体柱在等规水相流动相条件下分离类固醇进行了比较。只需改变温度，而不是改变流动相组成，就可以优化分离。(C)2008爱思唯尔B.V.保留所有权利。
One of the recent major improvements of HPLC was the introduction of monolithic silica columns, which have the advantage of faster separation and lower back pressure as compared to common silica beads. Here, we present an interesting alternative to such reversed-phase monolithic columns by a convenient coupling route of a thermo-responsive polymer to hydrophilic silica monoliths. Poly(N-isopropylacrylamide) (PNIPAM) was polymerized in solution via a reversible addition fragmentation chain transfer (RAFT) polymerization technique and coupled then in situ onto an amino-modified silica monolithic column. These columns were compared with RP-18 monolithic columns in the separation of steroids under isocratic condition in aqueous mobile phase. Separation is optimized just by changing the temperature, instead of changing the mobile phase composition. (C) 2008 Elsevier B.V. All rights reserved.