Housefly Phormicin inhibits Staphylococcus aureus and MRSA by disrupting biofilm formation and altering gene expression in vitro and in vivo.
Housefly Phormicin inhibits Staphylococcus aureus and MRSA by disrupting biofilm formation and altering gene expression in vitro and in vivo.
复制标题
家蝇Phormicin通过破坏生物膜形成和改变体内外基因表达来抑制金黄色葡萄球菌和MRSA。
DOI：
10.1016/j.ijbiomac.2020.11.096
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发表时间：
2020-11
影响因子：
8.2
通讯作者：
B. Wang;Yang Yao;Peng-Wei Wei-Peng-Wei-Wei-2026663216;Chao-Rong Song;Shan Wan;Suwen Yang;Guiming Zhu;Hong Mei Liu
中科院分区：
文献类型：
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作者：
B. Wang;Yang Yao;Peng-Wei Wei-Peng-Wei-Wei-2026663216;Chao-Rong Song;Shan Wan;Suwen Yang;Guiming Zhu;Hong Mei Liu
The increasing drug resistance of pathogenic bacteria is a crisis that threatens public health. Antimicrobial peptides (AMPs) have been suggested to be potentially effective alternatives to solve this problem. Here, we tested housefly Phormicin-derived peptides for effects onStaphylococcus aureusand methicillin-resistantStaphylococcus aureus(MRSA) infections in vitro and in vivo.A decreased bacterial load of MRSA was observed in the mouse scald model after treatment with Phormicin and in the positive control group (vancomycin). A mouse scrape model indicated that Phormicin helps the host fight drug-resistant MRSA infections. The protective effect of Phormicin on MRSA was confirmed in theHermetia illucenslarvae model. Phormicin also disrupted the formation ofS. aureusand MRSA biofilms. Furthermore, this effect coincided with the downregulation of biofilm formation-related gene expression (agrC,sigB,RNAIII,altA,rbf,hla,hld,gehandpsmɑ). Notably, virulence genes and several regulatory factors were also altered by Phormicin treatment. Based on these findings, housefly Phormicin helps the host inhibit MRSA infection through effects on biofilm formation and related gene networks. Therefore, housefly Phormicin potential represents a candidate agent for clinical MRSA chemotherapy.
