Identification of miRNAs and Their Target Genes Using Deep Sequencing and Degradome Analysis in Trifoliate Orange [Poncirus trifoliate (L.) Raf]
Identification of miRNAs and Their Target Genes Using Deep Sequencing and Degradome Analysis in Trifoliate Orange [Poncirus trifoliate (L.) Raf]
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使用三叶橙 [Poncirus trifoliate (L.) Raf] 的深度测序和降解组分析鉴定 miRNA 及其靶基因
DOI：
10.1007/s12033-011-9439-x
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发表时间：
2012-06
影响因子：
2.6
通讯作者：
Hu, Chun-Gen
中科院分区：
文献类型：
--
作者：
Zhang, Jin-Zhi;Ai, Xiao-Yan;Guo, Wen-Wu;Peng, Shu-Ang;Deng, Xiu-Xin;Hu, Chun-Gen
为了鉴定柑橘中新的和保守的miRNAs，将小RNA文库与基因芯片相结合，对早熟三叶橙(三叶橙的早花突变体)进行了深度测序，共获得了114个保守的miRNAs，分属于38个科155个新的miRNAs。在新发现的miRNAs中还发现了39个保守的miRNAs和27个新的miRNAs的miRNA STAR序列，为miRNAs的存在提供了进一步的证据。通过降解基因组测序，分别鉴定了172个和149个基因是保守的miRNAs和新的miRNAs的靶标。GO和KEGG注释显示，高排名的miRNA靶基因是那些与生物和代谢过程有关的基因。为了鉴定这些在柑橘幼体和成体发育阶段表达的miRNAs，利用商业基因芯片和实时荧光定量聚合酶链式反应对这些miRNAs的表达谱进行了进一步分析。结果表明，一些miRNAs在成虫期比幼虫期表达下调。对一些miRNAs和相应靶基因的表达模式进行了详细的比较，发现它们之间存在负相关，但很少有正相关。
To identify novel as well as conserved miRNAs in citrus, deep sequencing of small RNA library combined with microarray was performed in precocious trifoliate orange (an early flowering mutant of trifoliate orange, Poncirus trifoliata L. Raf.), resulting in the obtainment of a total of 114 conserved miRNAs belonging to 38 families and 155 novel miRNAs. The miRNA star sequences of 39 conserved miRNAs and 27 novel miRNAs were also discovered among newly identified miRNAs, providing additional evidence for the existence of miRNAs. Through degradome sequencing, 172 and 149 genes were identified as targets of conserved miRNAs and novel miRNAs, respectively. GO and KEGG annotation revealed that high ranked miRNA-target genes were those implicated in biological and metabolic processes. To characterize those miRNAs expressed at the juvenile and adult development stages of citrus, further analysis on the expression profiles of these miRNAs through hybridizing the commercial microarray and real-time PCR was performed. The results revealed that some miRNAs were down-regulated at adult stage compared with juvenile stage. Detailed comparison of the expression patterns of some miRNAs and corresponding target genes revealed the negative correlation between them, while few of them are positively correlated.
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影响因子：
2.4
作者：
Xiao-Meng Wu;Mei-Ya Liu;Qiang Xu;Wenwu Guo
通讯作者：
Xiao-Meng Wu;Mei-Ya Liu;Qiang Xu;Wenwu Guo
小麦 (Triticum aestivum L.) 的 microRNA 的克隆和表征
影响因子：
12.3
作者：
Yao Y;Guo G;Ni Z;Sunkar R;Du J;Zhu JK;Sun Q
通讯作者：
Sun Q
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
4.6
作者：
Wu, Gang;Poethig, R. Scott
通讯作者：
Poethig, R. Scott
柑橘中 27 个保守 microRNA 的鉴定和表征。
影响因子：
4.3
作者：
Song, Changnian;Fang, Jinggui;Li, Xiaoying;Liu, Hong;Chao, C. Thomas
通讯作者：
Chao, C. Thomas