An extracellular esterase of Aspergillus awamori
An extracellular esterase of Aspergillus awamori
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泡盛曲霉胞外酯酶
DOI：
--
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发表时间：
1990
影响因子：
2.8
通讯作者：
M. Linko
中科院分区：
文献类型：
--
作者：
M. Sundberg;K. Poutanen;P. Markkanen;M. Linko
用离子交换层析法从泡盛曲霉VTT-D-75028的培养滤液中分离得到一种胞外酯酶。纯化倍数为200倍，回收率为51%。分离的酶具有150，000的Mr，并且由三个相同的亚基组成。其等电点为3.7-3.8。纯化后的酯酶在65 ℃（pH5.3）下至少保持2天的活性。该酶水解脂肪族和芳香族酯，优先水解丁酸酯，并暂时归类为羧酸酯酶（EC 3.1.1.1）。
An extracellular esterase was isolated from the culture filtrate of Aspergillus awamori VTT-D-75028 by ion-exchange chromatography. Purificaiton of the preparation was 200-fold and recovery 51%. The isolated enzyme had a M r of 150,000 and consisted of three identical subunits. Its isoelectric point was 3.7-3.8. The purified esterase was very stable and retained all its activity for at least 2 days at 65 o C (pH 5.3). The enzyme hydrolyzed both aliphatic and aromatic esters, preferentially butyrates, and was tentatively classified as a carboxylic esterase (EC 3.1.1.1)