Different roles of odontoblasts and fibroblasts in immunity
Different roles of odontoblasts and fibroblasts in immunity
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DOI：
10.1177/154405910808700304
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发表时间：
2008-03-01
影响因子：
7.6
通讯作者：
Farges, J. -C.
中科院分区：
文献类型：
--
作者：
Staquet, M. -J.;Durand, S. H.;Farges, J. -C.
成牙本质细胞和成纤维细胞被怀疑影响在龋齿过程中逐渐入侵人类牙齿的微生物在牙髓中触发的先天免疫反应。为了确定它们对口腔病原体的反应是否不同，我们对成牙本质细胞样细胞和牙髓成纤维细胞对TLR2、TLR3和TLR4特异性激动剂(分别是脂磷壁酸[LTA]、双链RNA和脂多糖[LPS])的反应进行了系统的比较分析。细胞对这些激动剂的反应是通过差异上调趋化因子基因的表达。因此，LTA只在成牙本质细胞样细胞中增加CXCL2和CXCL10，而在成纤维细胞中只增加CCL7、CCL26和CXCL11。与对照组相比，刺激培养上清液增加了未成熟树突状细胞的迁移，成牙本质细胞样细胞是比成纤维细胞更有效的诱导剂。对这些数据的分析表明，成牙本质细胞和牙髓成纤维细胞对侵入牙髓组织的口腔微生物的先天免疫反应不同。缩写：TLR，Toll样受体；LTA，脂磷壁酸；LPS，脂多糖；dsRNA，双链核糖核酸；Poly(I：C)，多肌苷脱氧胞苷；DC，树突状细胞。
Odontoblasts and fibroblasts are suspected to influence the innate immune response triggered in the dental pulp by micro-organisms that progressively invade the human tooth during the caries process. To determine whether they differ in their responses to oral pathogens, we performed a systematic comparative analysis of odontoblast-like cell and pulp fibroblast responses to TLR2-, TLR3-, and TLR4-specific agonists ( lipoteichoic acid [ LTA], double-stranded RNA, and lipopolysaccharide [ LPS], respectively). Cells responded to these agonists by differential upregulation of chemokine gene expression. CXCL2 and CXCL10 were thus increased by LTA only in odontoblast- like cells, while LPS increased CCL7, CCL26, and CXCL11 only in fibroblasts. Supernatants of stimulated cultures increased migration of immature dendritic cells compared with controls, odontoblast- like cells being more potent attractants than fibroblasts. Analysis of these data suggests that odontoblasts and pulp fibroblasts differ in their innate immune responses to oral micro-organisms that invade the pulp tissue. Abbreviations: TLR, Toll-like receptor; LTA, lipoteichoic acid; LPS, lipopolysaccharide; dsRNA, double-stranded ribonucleic acids; Poly( I: C), polyinosinedeoxycytidylic acid; DC, dendritic cell.