Characterization of the protein product of bcl-2, the gene involved in human follicular lymphoma.
Characterization of the protein product of bcl-2, the gene involved in human follicular lymphoma.
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发表时间：
1987
期刊：
影响因子：
8
通讯作者：
Y. Tsujimoto;N. Ikegaki;C. Croce
中科院分区：
文献类型：
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作者：
Y. Tsujimoto;N. Ikegaki;C. Croce
利用兔抗-半乳糖苷酶/bcl-2融合蛋白多克隆抗血清，从人b细胞提取物中特异性免疫沉淀出与bcl-2 α蛋白大小相对应的蛋白，该蛋白在兔网织细胞裂解液中进行体外转录和体外翻译。与V8蛋白酶的肽图谱证实了该蛋白为人bcl-2 α。细胞蛋白亚细胞分离和免疫沉淀表明bcl-2 α蛋白与细胞膜相关。
A protein corresponding in size to the bcl-2 alpha protein obtained after in vitro transcription and in vitro translation in a rabbit reticulocyte lysate was specifically immunoprecipitated from a human B-cell extract using rabbit polyclonal antiserum raised against a beta-galactosidase/bcl-2 fusion protein. Peptide mapping with V8 protease confirmed the identity of this protein as human bcl-2 alpha. Subcellular fractionation of cellular protein followed by immunoprecipitation showed that the bcl-2 alpha protein is associated with the cell membrane.