QUANTITATION OF INDUCED MUTATION IN DICTYOSTELIUM-DISCOIDEUM CHARACTERIZATION AND USE OF A METHANOL-RESISTANCE MUTATION ASSAY
QUANTITATION OF INDUCED MUTATION IN DICTYOSTELIUM-DISCOIDEUM CHARACTERIZATION AND USE OF A METHANOL-RESISTANCE MUTATION ASSAY
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DOI：
10.1016/0165-1161(80)90176-4
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
DEERING, RA
中科院分区：
文献类型：
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作者：
PODGORSKI, G;DEERING, RA
一种基于盘形藻对3%甲醇抗性的正向突变的检测方法，可以对物理和化学制剂处理后的诱导突变进行定量。该方法的特点是：甲醇抗性(MeOHr)细胞在广泛的平板细胞密度范围内均匀恢复，在表达期间没有甲醇的情况下，甲醇敏感细胞和甲醇抗性细胞的生长速度相等，突变数量随着表达时间的变化而达到平台期。4种诱变剂在10%的存活率水平下，N-甲基-N‘’-亚硝胺的诱变效果最好，其次是~(60)Co-γ射线、紫外光和甲磺酸甲酯。
An assay based on forward mutation of D. discoideum to 3% methanol resistance allows quantitation of induced mutation following treatment with physical and chemical agents. Properties of this assay are: uniform recovery of methanol-resistant (MeOHr) cells over a wide range of plated cell densities, equal growth rates of methanol-sensitive and methanol-resistant cells in the absence of methanol during the expression period and the attainment of plateau in the number of mutants as a function of expression time. When 4 mutagens were tested with this system and compared at the 10% survival level, N-methyl-N''-nitrosoguanidine was the most effective for mutation induction, followed by 60Co-.gamma.-rays, UV light and methyl methanesulfonate.