Melatonin lowers excitability of guinea pig hippocampal neurons in vitro
Melatonin lowers excitability of guinea pig hippocampal neurons in vitro
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DOI：
10.1007/bf00611091
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发表时间：
1985-07
期刊：
影响因子：
--
通讯作者：
M. Zeise;P. Semm
中科院分区：
文献类型：
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作者：
M. Zeise;P. Semm
用细胞内和细胞外记录方法研究了褪黑素（N-乙酰基-5-甲氧基色胺; MEL）对离体豚鼠海马CA 3区神经元和齿状颗粒细胞的作用。Mel（1-10 mmol/l）有以下作用：1.对重复突触刺激的反应发生显著改变;双电击易化（20 ms间隔）结果表明：1.动作电位的频率降低，1.动作电位的频率降低，2.动作电位的频率降低，3.动作电位的持续时间增加，4.动作电位的触发阈值正移结果表明：（1）Bicuculline可使IPSPs延长，其分流能力增强;（2）Bicuculline可可逆性地消除IPSPs引起的重复峰电位，这些作用的共同点是降低细胞兴奋性。结论：MEL可能影响癫痫发作活动，应进一步研究作为潜在的抗惊厥药。
Action of melatonin (N-acetyl-5-methoxytryptamine; MEL) on guinea pig hippocampal cells (CA3 neurons and dentate granule cells) were studied in vitro using both extra- and intracellular recording. MEL (1–10 mmol/l) had the following effects:1.Response to repetitive synaptic stimulation was changed drastically: Double shock facilitation (20 ms interval) turned into depression and stimulus trains of a frequency as low as 1 Hz led to a drastic reduction of the response.2.Membrane potential was hyperpolarized.3.Duration of action potential was strongly increased.4.Threshold for the triggering of action potentials was shifted to more positive levels.5.IPSPs were prolonged and their shunting power enhanced.6.Repetitive spiking elicited by the application of bicuculline was reversibly abolished.All these effects had in common that cell excitability was lowered. It is concluded that MEL might influence epileptic seizure activity and should be further investigated as potential anticonvulsant.