Physical activity and circulating endothelial progenitor cells: an intervention study
Physical activity and circulating endothelial progenitor cells: an intervention study
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DOI：
10.1111/j.1365-2362.2012.02670.x
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发表时间：
2012-09-01
影响因子：
5.5
通讯作者：
Casini, Alessandro
中科院分区：
文献类型：
--
作者：
Cesari, Francesca;Sofi, Francesco;Casini, Alessandro
欧洲临床投资杂志 2012； 42 (9): 927932 摘要背景 评估个性化体育活动计划对超重和肥胖受试者的体重和循环 (CPC) 以及内皮祖细胞 (EPC) 的影响。材料和方法 对总共 80 名超重和肥胖受试者进行身体成分测量、心肺测试、最大摄氧量 (VO2max) 最大压力运动测试以及一系列生化分析。使用流式细胞术测定CPC和EPC，CPC定义为CD34+、CD133+和CD34+/CD133+，EPC定义为CD34+KDR+、CD133+KDR+和CD34+CD133+KDR+。结果 在计划结束时，我们将人群分为两组：合规个体（A 组，n = 47）和不合规个体（B 组，n = 33）。 A 组报告体重显着减少 3.1% (P < 0.0001)，脂肪量显着减少 4.4% (P < 0.0001)，而 B 组在 T1 时脂肪量百分比增加 1.5%。 A组中，T1时观察到CPC和EPC循环水平呈上升趋势，三种类型的EPC均达到统计学显着性。相反，B组的CPC和EPC没有显着增加。此外，A 组脂肪量的减少与 CD133+/KDR+ EPC 的增加之间存在显着相关性（r = 0.50；P = 0.04）。结论 三个月的体力活动显着改善了人体测量结果。观察到依从性个体中 EPC 数量增加对体重减轻产生有益影响。
Eur J Clin Invest 2012; 42 (9): 927932 Abstract Background To assess the effect of a personalized physical activity programme on weight and circulating (CPC) and endothelial progenitor cells (EPC) in overweight and obese subjects. Materials and methods Anthropometric measurements with body composition, cardiopulmonary test, maximal stress exercise test with maximal oxygen uptake (VO2max) and a series of biochemical analyses were taken before (T0) and after 3 months of physical activity (T1) in a total of 80 overweight and obese subjects. CPC and EPC were determined using flow cytometry and were defined as CD34+, CD133+ and CD34+/CD133+ for CPC and CD34+KDR+, CD133+KDR+ and CD34+CD133+KDR+ for EPC. Results At the end of the programme, we divided the population into two groups, compliant individuals (group A, n = 47) and noncompliant individuals (group B, n = 33). Group A reported significant reductions of weight by 3.1% (P < 0.0001) and fat mass by 4.4% (P < 0.0001), while group B showed a percentage of increase in fat mass by 1.5% at T1. In group A, a trend of increase at T1 for circulating levels of CPC and EPC was observed, reaching the statistical significance for all the three types of EPC. On the contrary, group B showed no significant increase in CPC and EPC. Furthermore, a significant correlation between decrease in fat mass and increase in CD133+/KDR+ EPC was reported in group A (r = 0.50; P = 0.04). Conclusion Three months of physical activity significantly improved anthropometric measurements. A beneficial effect of increased number of EPC in compliant individuals, in relation to weight loss, was observed.