RECOGNITION BY VIRAL AND CELLULAR DNA-POLYMERASES OF NUCLEOSIDES BEARING BASES WITH NONSTANDARD HYDROGEN-BONDING PATTERNS
RECOGNITION BY VIRAL AND CELLULAR DNA-POLYMERASES OF NUCLEOSIDES BEARING BASES WITH NONSTANDARD HYDROGEN-BONDING PATTERNS
复制标题
DOI：
10.1073/pnas.92.14.6329
复制
发表时间：
1995-07-03
影响因子：
11.1
通讯作者：
BENNER, SA
中科院分区：
文献类型：
--
作者：
HORLACHER, J;HOTTIGER, M;BENNER, SA
DNA聚合酶（pols）催化双链体寡核苷酸的模板定向合成的能力，所述双链体寡核苷酸含有在带有5-羧基端的核苷酸之间的非标准沃森-克里克碱基对。已经研究了（2，4-二氨基嘧啶）杂环（d κ）和带有脱氧黄嘌呤核苷（dX）或N-1-甲基氧甲霉素B（pi）的核苷酸，kappa-X和kappa-pi碱基对通过与连接AT和GC碱基对的氢键模式不同且不包括连接AT和GC碱基对的氢键模式连接，来自人类免疫缺陷病毒1型（HIV-1）的逆转录酶以良好的保真度将dXTP掺入模板中与d κ相对的寡核苷酸中，HIV-1逆转录酶也在模板中掺入与dX相对的dKTP，在模板中有d pi的情况下，用HIV逆转录酶没有观察到dKTP的掺入，来自大肠杆菌的DNA pol I的Klenow片段在模板中不掺入与dX相对的dKTP，但掺入与dKTP相对的dXTP，牛DNA pol α，β，并且，dXTP既不接受与d kappa相反的dXTP，也不接受与d pi相反的d kappa TP。DNA聚合酶α和β（但不是β）将dkappa TP相反DX在模板中，但停止延长后，将一个单一的额外的基地，这些结果进行了讨论，在光的晶体结构聚合酶β和一般的考虑如何必须与传入的碱基对忠实地复制遗传信息。
The ability of DNA polymerases (pols) to catalyze the template-directed synthesis of duplex oligonucleotides containing a nonstandard Watson-Crick base pair between a nucleotide bearing a 5-(2,4-diaminopyrimidine) heterocycle (d kappa) and a nucleotide bearing either deoxyxanthosine (dX) or N-1-methyloxoformycin B (pi) has been investigated, The kappa-X and kappa-pi base pairs are joined by a hydrogen bonding pattern different from and exclusive of those joining the AT and GC base pairs, Reverse transcriptase from human immunodeficiency virus type 1 (HIV-1) incorporates dXTP into an oligonucleotide opposite d kappa in a template with good fidelity, With lower efficiency and fidelity, HIV-1 reverse transcriptase also incorporates d kappa TP opposite dX in the template, With d pi in the template, no incorporation of dKTP was observed with HIV reverse transcriptase, The Klenow fragment of DNA pol I from Escherichia coli does not incorporate d kappa TP opposite dX in a template but does incorporate dXTP opposite d kappa, Bovine DNA pols alpha, beta, and epsilon accept neither dXTP opposite d kappa nor d kappa TP opposite d pi. DNA pols alpha and epsilon (but not beta) incorporate d kappa TP opposite dX in a template but discontinue elongation after incorporating a single additional base, These results are discussed in light of the crystal structure for pol beta and general considerations of how polymerases must interact with an incoming base pair to faithfully copy genetic information.